ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/052/SRR26779852/SRR26779852.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/055/SRR26779855/SRR26779855.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/035/SRR26779835/SRR26779835.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/032/SRR26779832/SRR26779832.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/046/SRR26779846/SRR26779846.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/044/SRR26779844/SRR26779844.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/054/SRR26779854/SRR26779854.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/033/SRR26779833/SRR26779833.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/050/SRR26779850/SRR26779850.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/043/SRR26779843/SRR26779843.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/048/SRR26779848/SRR26779848.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/041/SRR26779841/SRR26779841.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/037/SRR26779837/SRR26779837.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/042/SRR26779842/SRR26779842.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/039/SRR26779839/SRR26779839.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/034/SRR26779834/SRR26779834.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/045/SRR26779845/SRR26779845.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/047/SRR26779847/SRR26779847.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/053/SRR26779853/SRR26779853.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/051/SRR26779851/SRR26779851.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/038/SRR26779838/SRR26779838.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/036/SRR26779836/SRR26779836.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/040/SRR26779840/SRR26779840.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR267/049/SRR26779849/SRR26779849.fastq.gzprimary200OKGenomicsSystems Neuroscience, D-HEST, ETH Zürichhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1037562Mus musculusWe recently reported that the loss of the microRNA cluster miR-379-410 leads to hypersocial behavior and anxiety in mice. With this study, we show that ablating miR-379-410 in excitatory neurons of the postnatal mouse hippocampus recapitulates hypersociability, but not anxiety. At the cellular level, miR-379-410 loss in excitatory neurons leads to increased excitatory synaptic transmission and upregulation of an actomyosin gene network. Re-expression of three cluster miRNAs, as well as pharmacological inhibition of the actomyosin activator ROCK, was sufficient to reinstate normal sociability in miR-379-410 knockout mice. Our results unveil a novel microRNA-actomyosin pathway involved in the control of sociability. Overall design: To investigate whether the knockout of miR379-410 miRNA cluster in glutamatergic neurons of the hippocampus drives differential gene expression, we established genetically modified mice with Cre-driven conditional knockout (cKO) of this cluster in Emx1 positive cells. We then performed gene expression profiling analysis using data obtained from RNA-seq of 6 different animals per genotype (6 wild type and 6 cKO mice).xref:PubMed:38968074ENAfalsemiRNA-mediated inhibition of an actomyosin network in hippocampal pyramidal neurons restricts sociability in adult male micemiRNA-mediated inhibition of an actomyosin network in hippocampal pyramidal neurons restricts sociability in adult male mice2025-09-242024-05-07PRJNA1037562GSE2474391009038968074