ENAapplication/xmlftp://primary200OKGenomicsNIDDKhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1043809Homo sapiensThe study goal was to apply spatial transcriptomics to identify candidate stromal drivers of benign prostatic hyperplasia (BPH). Laser microdissection (LCM) with RNA-seq of the stroma adjacent to hyperplastic and budding BPH ducts identified secreted factors including IGF1 and CXCL13. Single-cell RNA sequencing (scRNA-seq) revealed their co-expression from BPH fibroblasts. Additional studies sought to define similarities to embryonic prostate development, as well as therapeutic applications. Subjects were those who underwent prostatectomy for prostate or bladder cancer, with concurrent BPH, and who consented to use of surplus tissue for research studies. LCM/RNA-seq studies included 5 patients, and scRNA-seq studies included 1 patient. Phenotypic data submitted to this study include... (for more see dbGaP study page.)ENAProfiling, Prostatic Adenoma, Benign Prostatic Hyperplasia, Culture Techniques, Hyperplasia, Transcriptome, AU023367, determination, Adenomas, Benign Prostatic Hyperplasias, Monitorings, Gene, Prostatic Hypertrophy, Xt, Cell, add, Gene Expression Profilings, Gene Expression, Differential Display, Transcript Expression Analysis, mRNA Differential Displays, benign prostatic hyperplasia., Transcript Expression Analyses, Gene Expression Pattern Analysis, Benign, GLI3-190, Prostatic Adenomas, Hyperplasias, chemical analysis, Gene Expression Monitorings, Analysis, Profilings, Benign Prostatic Hypertrophy, Cell Culture, mRNA Differential Display, Bph, BPH, Prostatic Hyperplasias, Hypertrophies, Culture Technique, Hypertrophy, Gene Expression Monitoring, Transcriptome Profiling, Analyses, mRNA, Adenoma, GLI3FL, Transcriptome Profilings, Benign Prostatic, AI854843, Tissue, Transcriptomics, Differential Displays, benign, Transcript Expression, Transcriptome Analysis, Pdn, Transcriptome Analyses, benign prostatic hyperplasia, Cell Culture Technique, Monitoring, Gene Expression Analysis, Prostatic Hypertrophies, Gene Expression Analyses, Prostatic Hyperplasia, Cell Cultures, Prostatic, Expression Analysis, assay, Expression Analyses, prostatic hyperplasiaProfiling, Prostatic Adenoma, Benign Prostatic Hyperplasia, Culture Techniques, Hyperplasia, Transcriptome, AU023367, determination, Adenomas, Benign Prostatic Hyperplasias, Monitorings, Gene, Prostatic Hypertrophy, Xt, Cell, add, Gene Expression Profilings, Gene Expression, Differential Display, Transcript Expression Analysis, mRNA Differential Displays, benign prostatic hyperplasia., Transcript Expression Analyses, Gene Expression Pattern Analysis, Benign, GLI3-190, Prostatic Adenomas, Hyperplasias, chemical analysis, Gene Expression Monitorings, Analysis, Profilings, Benign Prostatic Hypertrophy, Cell Culture, mRNA Differential Display, Bph, BPH, Prostatic Hyperplasias, Hypertrophies, Culture Technique, Hypertrophy, Gene Expression Monitoring, Transcriptome Profiling, Analyses, mRNA, Adenoma, GLI3FL, Transcriptome Profilings, Benign Prostatic, AI854843, Tissue, Transcriptomics, Differential Displays, benign, Transcript Expression, Transcriptome Analysis, Pdn, Transcriptome Analyses, benign prostatic hyperplasia, Cell Culture Technique, Monitoring, Gene Expression Analysis, Prostatic Hypertrophies, Gene Expression Analyses, Prostatic Hyperplasia, Cell Cultures, Prostatic, Expression Analysis, assay, Expression Analyses, prostatic hyperplasiafalseBPH Tissues for Cell Culture and Analysis - Spatial transcriptomics identifies candidate stromal drivers of benign prostatic hyperplasiaBPH Tissues for Cell Culture and Analysis - Spatial transcriptomics identifies candidate stromal drivers of benign prostatic hyperplasia2025-09-242023-12-25PRJNA10438099606