{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/089/SRR26965489/SRR26965489_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/088/SRR26965488/SRR26965488_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/087/SRR26965487/SRR26965487_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/090/SRR26965490/SRR26965490_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/095/SRR26965495/SRR26965495_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/094/SRR26965494/SRR26965494_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/092/SRR26965492/SRR26965492_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/091/SRR26965491/SRR26965491_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/095/SRR26965495/SRR26965495_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/088/SRR26965488/SRR26965488_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/089/SRR26965489/SRR26965489_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/091/SRR26965491/SRR26965491_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/092/SRR26965492/SRR26965492_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/087/SRR26965487/SRR26965487_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/093/SRR26965493/SRR26965493_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/090/SRR26965490/SRR26965490_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/094/SRR26965494/SRR26965494_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR269/093/SRR26965493/SRR26965493_1.fastq.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Shenzhen Institutes of Advanced Technology"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA1045625"],"scientific_name":["Drosophila melanogaster"],"tag":["xref:PubMed:39747148"],"long_description":["Long noncoding RNAs known as roX (RNA on X) are crucial for male development in Drosophila, as their loss leads to male lethality from the late larval stages. While roX RNAs are recognized for their role in sex-chromosome dosage compensation, ensuring balanced expression of X-linked genes in both sexes, their potential influence on autosomal gene regulation remains unexplored. Using an integrative multi-omics approach, we revealed that roX RNAs not only govern the X chromosome but also target genes on autosomes that lack male-specific lethal (MSL) complex occupancy, together with Polycomb repressive complexes (PRCs). We observed that roX RNAs colocalize with MSL proteins on the X chromosome and PRC components on autosomes. Intriguingly, loss of roX function reduces X-chromosomal H4K16ac levels and autosomal H3K27me3 levels. Correspondingly, X-linked genes display reduced expression, whereas many autosomal genes exhibit elevated expression upon roX loss. Our findings propose a dual role for roX RNAs: activators of X-linked genes and repressors of autosomal genes, achieved through interactions with MSL and PRC complexes, respectively. This study uncovers the unconventional epigenetic repressive function of roX RNAs. Overall design: Genomic binding profiles of H3K27me3 in roX1 roX2 double knockout (roX-KO) male larvae and their wild type counterparts were generated by ChIP-seq."],"repository":["ENA"],"description_synonyms":["HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, ChIP-Chip, Chromatin Immuno-precipitation, 60S ribosomal protein L8 histidine hydroxylase, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, ChIP Sequencing, CLIP-Seq, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP-PET, ChIP-Exo, Concept, MINA53, bHLHd3, Ribosomal oxygenase NO66, Nucleolar protein 66, MAD6, Role Concept, Roles, MAPJD, Chromatin Immunoprecipitation Sequencing-Chip, Role, Concepts, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Histone lysine demethylase NO66, ROX, NO52, Rox, hsNO66, xmnt, Chromatin Immunoprecipitation Sequencing Chip, Chromatin Immuno precipitation Sequencing, Histone lysine demethylase MINA, Nucleolar protein 52, Myc-associated protein with JmjC domain, Repression, Epigenetic, ChIP, Chromatin Immunoprecipitation Paired End Tag, Chromatin Immuno Precipitation Paired End Tag, MDIG, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, Chromatin Immunoprecipitation, Ribosomal oxygenase MINA, Chromatin Immuno-precipitation Sequencing, ChIP Exonuclease, rox, ChIP-Seq, Sequencing, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, NO66, 1.14.11.27, ChIA-PET., Chromatin Immuno-Precipitation Paired-End Tag, Role Concepts, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Mineral dust-induced gene protein, MYC-induced nuclear antigen, 1.14.11.-, MXD6, ChIP-Exonuclease, 60S ribosomal protein L27a histidine hydroxylase"],"name_synonyms":["HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, ChIP-Chip, Chromatin Immuno-precipitation, 60S ribosomal protein L8 histidine hydroxylase, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, ChIP Sequencing, CLIP-Seq, Assay for Transposase-Accessible Chromatin Using Sequencing, ChIP-PET, ChIP-Exo, Concept, MINA53, bHLHd3, Ribosomal oxygenase NO66, Nucleolar protein 66, MAD6, Role Concept, Roles, MAPJD, Chromatin Immunoprecipitation Sequencing-Chip, Role, Concepts, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Histone lysine demethylase NO66, ROX, NO52, Rox, hsNO66, xmnt, Chromatin Immunoprecipitation Sequencing Chip, Chromatin Immuno precipitation Sequencing, Histone lysine demethylase MINA, Nucleolar protein 52, Myc-associated protein with JmjC domain, Repression, Epigenetic, ChIP, Chromatin Immunoprecipitation Paired End Tag, Chromatin Immuno Precipitation Paired End Tag, MDIG, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, Chromatin Immunoprecipitation, Ribosomal oxygenase MINA, Chromatin Immuno-precipitation Sequencing, ChIP Exonuclease, rox, ChIP-Seq, Sequencing, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, NO66, 1.14.11.27, ChIA-PET., Chromatin Immuno-Precipitation Paired-End Tag, Role Concepts, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Mineral dust-induced gene protein, MYC-induced nuclear antigen, 1.14.11.-, MXD6, ChIP-Exonuclease, 60S ribosomal protein L27a histidine hydroxylase"],"additional_accession":[]},"is_claimable":false,"name":"A noncanonical role of roX RNAs in autosomal epigenetic repression [ChIP-seq]","description":"A noncanonical role of roX RNAs in autosomal epigenetic repression [ChIP-seq]","dates":{"last_updated":"2025-09-24","first_public":"2024-11-21"},"accession":"PRJNA1045625","cross_references":{"GEO":["GSE248698"],"taxon":["7227"],"PubMed":["39747148"]}}