ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/059/SRR27682259/SRR27682259_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/058/SRR27682258/SRR27682258_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/056/SRR27682256/SRR27682256_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/061/SRR27682261/SRR27682261_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/060/SRR27682260/SRR27682260_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/058/SRR27682258/SRR27682258_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/057/SRR27682257/SRR27682257_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/061/SRR27682261/SRR27682261_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/060/SRR27682260/SRR27682260_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/059/SRR27682259/SRR27682259_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/057/SRR27682257/SRR27682257_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR276/056/SRR27682256/SRR27682256_1.fastq.gzprimaryOK200GenomicsChildren’s Hospital of Fudan Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1067600Mus musculusBased on previous experiments showing that Alisol B could regulate GnRH secretion from GT1-7 cells, cellular transcriptome sequencing was used to investigate the potential mechanism by which Alisol B regulates GnRH secretion from GT1-7 cells. Overall design: To investigate the effect of Alisol B on gene expression in GT1-7 cells, when the cells reached 70-80% confluence, they were starved in serum-free medium for 12 hours. Afterward, they were incubated for 24 hours with or without 0.125μM Alisol B. We then performed gene expression profiling analysis using data obtained from RNA-seq of the Alisol B intervention group and the control group cells with three replicates each. Differential gene expressions analysis of RNA-seq data of the Alisol B intervention group and the control group cells with three replicates each.ENAfalseEffect of Alisol B on gene expression in GT1-7 cellsEffect of Alisol B on gene expression in GT1-7 cells2025-09-242025-04-14PRJNA1067600GSE25387510090