ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/062/SRR27840362/SRR27840362_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/061/SRR27840361/SRR27840361_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/063/SRR27840363/SRR27840363_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/064/SRR27840364/SRR27840364_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/066/SRR27840366/SRR27840366_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/062/SRR27840362/SRR27840362_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/064/SRR27840364/SRR27840364_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/061/SRR27840361/SRR27840361_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/065/SRR27840365/SRR27840365_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/065/SRR27840365/SRR27840365_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/066/SRR27840366/SRR27840366_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR278/063/SRR27840363/SRR27840363_2.fastq.gzprimaryOK200GenomicsPopulation Health Sciences, UT Health Science Center at San Antoniohttps://www.ebi.ac.uk/ena/browser/view/PRJNA1072101Homo sapiensxref:PubMed:39258975We examined the significance and therapeutic potential of targeting PELP1 in Hepatocellular carcinoma. Total RNA were isolated from HEP3B-control Sh, and HEP3B-PELP1-Sh cells using RNeasy mini kit, and total RNA was isolated for RNA-seq analysis. Our results demonstrated that PELP1 knockdown regulates pathways related to inflammation and interferon signaling and downregulates several pathways including MYC and E2F. Overall design: Total RNA were isolated from Hep3B-control Sh, and Hep3B-PELP1-Sh using RNeasy mini kit (Qiagen). For whole-genome transcriptome profiling, 2 libraries (3 replicates from each group) were generated using a TruSeq Stranded mRNA Library Preparation kit according to the manufacturer’s protocol (Illumina Inc.). Samples were sequenced on the Illumina HiSeq 3000 platform (Illumina Inc.) using the 50 base-pair single-read (50SR) sequencing module.ENAfalseThe therapeutic potential and significance of the PELP1 signaling pathway in hepatocellular carcinomaThe therapeutic potential and significance of the PELP1 signaling pathway in hepatocellular carcinoma2025-09-242024-10-18PRJNA1072101GSE254860960639258975