ENAGenomicsShoemaker Lab, Agronomy, Iowa State Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA107501Glycine maxxref:PubMed:19678937This study was designed to identify candidate genes associated with iron efficiency in soybeans. Two genotypes, Clark (PI548553) and IsoClark (PI547430), were grown in both iron sufficient (100uM Fe(NO3)3) and iron deficient (50uM Fe(NO3)3) hydroponics conditions. The second trifoliate was harvested for RNA extraction for the microarray experiment. Candidate genes were identified by comparing gene expression profiles within genotypes between the two iron growth conditions. Keywords: Stress Response Overall design: This experiment was designed to compare expression profiles of Clark grown in iron sufficient and deficient iron conditions and of IsoClark grown in the same conditions. Plants grown in iron sufficient conditions were used as controls and plants grown in iron deficient conditions were considered experimental. For the Clark genotype, There were two biological replicates of iron deficient plants, and three biological replicates of iron sufficient plants. The IsoClark genotype had three biological replicates for both iron sufficient and deficient conditions.ENASoybeans, portion of tissue, ferrum, hierro, simple tissue., determination, Iron, Soybean, Phaseolus max, Bean, soybeans, Soy Beans, Tissue, fer, Iron-56, hoja (Spanish, 葉 (Japanese, Fe, soybean, Soy Bean, cv. Wye, Eisen, Iron 56, exact), Glycine max, chemical analysis, tissue portion, Beans, 26Fe, assay, iron, Soysoybean, Soy Beans, Soy Bean, cv. Wye, Beans, Soybeans, soybeans., Phaseolus max, Soybean, Soy, Glycine max, BeanfalseGlycine maxAnalysis of Iron Deficiency in Soybean Leaf Tissue2025-09-242014-02-11PRJNA107501GSE10730384719678937