{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/031/SRR28260631/SRR28260631_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/025/SRR28260625/SRR28260625_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/023/SRR28260623/SRR28260623_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/029/SRR28260629/SRR28260629_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/022/SRR28260622/SRR28260622_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/021/SRR28260621/SRR28260621_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/027/SRR28260627/SRR28260627_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/027/SRR28260627/SRR28260627_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/024/SRR28260624/SRR28260624_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/031/SRR28260631/SRR28260631_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/030/SRR28260630/SRR28260630_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/021/SRR28260621/SRR28260621_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/024/SRR28260624/SRR28260624_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/032/SRR28260632/SRR28260632_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/030/SRR28260630/SRR28260630_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/026/SRR28260626/SRR28260626_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/028/SRR28260628/SRR28260628_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/022/SRR28260622/SRR28260622_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/028/SRR28260628/SRR28260628_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/026/SRR28260626/SRR28260626_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/025/SRR28260625/SRR28260625_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/032/SRR28260632/SRR28260632_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/029/SRR28260629/SRR28260629_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/023/SRR28260623/SRR28260623_2.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Southern Medical University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA1085296"],"scientific_name":["Homo sapiens"],"long_description":["Efficiency of HBG gene activation by the Cas12f/cgRNA system. In a doxycycline-inducible dCas12f-VPR knock-in (KI) HEK293T cell line, transiently transfected plasmids encoding U6, linear, and circular gRNAs targeting HBG1, and cgRNAs targeting mNeonGreen (negative control, NC) to activate HBG gene expression. RNA-seq analysis showed that cgRNAs exhibited increased efficiency in the target gene HBG1 and slight lower specificity when compared with U6 and linear gRNAs. Overall design: To define whether Cas12f/cgRNA system could enhance dCas12f-based endogenous gene activation, we transiently transfected different plasmids encoding gRNAs to activate HBG gene expression in the doxycycline-inducible dCas12f-VPR knock-in (KI) HEK293T cell line. Then we performed RNA-sequencing and compared our date with HBG gene expression from publicly available databases."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptomic profiling of HBG gene expression in HEK293T cell line","description":"Transcriptomic profiling of HBG gene expression in HEK293T cell line","dates":{"last_updated":"2025-09-24","first_public":"2024-03-30"},"accession":"PRJNA1085296","cross_references":{"GEO":["GSE261105"],"taxon":["9606"]}}