ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/031/SRR28260631/SRR28260631_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/025/SRR28260625/SRR28260625_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/023/SRR28260623/SRR28260623_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/029/SRR28260629/SRR28260629_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/022/SRR28260622/SRR28260622_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/021/SRR28260621/SRR28260621_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/027/SRR28260627/SRR28260627_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/027/SRR28260627/SRR28260627_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/024/SRR28260624/SRR28260624_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/031/SRR28260631/SRR28260631_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/030/SRR28260630/SRR28260630_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/021/SRR28260621/SRR28260621_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/024/SRR28260624/SRR28260624_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/032/SRR28260632/SRR28260632_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/030/SRR28260630/SRR28260630_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/026/SRR28260626/SRR28260626_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/028/SRR28260628/SRR28260628_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/022/SRR28260622/SRR28260622_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/028/SRR28260628/SRR28260628_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/026/SRR28260626/SRR28260626_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/025/SRR28260625/SRR28260625_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/032/SRR28260632/SRR28260632_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/029/SRR28260629/SRR28260629_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR282/023/SRR28260623/SRR28260623_2.fastq.gzprimaryOK200GenomicsSouthern Medical Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1085296Homo sapiensEfficiency of HBG gene activation by the Cas12f/cgRNA system. In a doxycycline-inducible dCas12f-VPR knock-in (KI) HEK293T cell line, transiently transfected plasmids encoding U6, linear, and circular gRNAs targeting HBG1, and cgRNAs targeting mNeonGreen (negative control, NC) to activate HBG gene expression. RNA-seq analysis showed that cgRNAs exhibited increased efficiency in the target gene HBG1 and slight lower specificity when compared with U6 and linear gRNAs. Overall design: To define whether Cas12f/cgRNA system could enhance dCas12f-based endogenous gene activation, we transiently transfected different plasmids encoding gRNAs to activate HBG gene expression in the doxycycline-inducible dCas12f-VPR knock-in (KI) HEK293T cell line. Then we performed RNA-sequencing and compared our date with HBG gene expression from publicly available databases.ENAfalseTranscriptomic profiling of HBG gene expression in HEK293T cell lineTranscriptomic profiling of HBG gene expression in HEK293T cell line2025-09-242024-03-30PRJNA1085296GSE2611059606