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Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1091422Mus musculusxref:EuropePMC:PMC11957694To systematically study the functions of metabolic genes in regulating anti-tumor immunity, we performed a series of in vivo metabolic-wide CRISPR screens in orthotopic lung tumor mice models. We constructed a metabolic-focused sgRNA library, established LLC-Cas9-library pools, and then performed in vivo CRISPR screens. We compared the PBS treated tumors in Rag1-/- mice or C57BL/6 mice with the input cell pool to check the functions of metabolic genes in tumor growth we compared the PBS treated tumors in C57BL/6 mice with the PBS treated tumors in Rag1-/- mice to check the functions of metabolic genes in regulating anti-tumor immunity we compared the anti-PD1 treated tumors in C57BL/6 mice with PBS treated tumors in C57BL/6 mice to check the functions of metabolic genes in modulating sensitivity to anti-PD1 treatment.ENAfalseMus musculusIn Vivo CRISPR Screening for metabolic regulators of immunotherapy2025-09-242024-05-11PRJNA109142210090