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Cornell Medicine in Qatar"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA1095688"],"long_description":["Given the increased predictions on interactome size and demand for protein function information, methods for detecting protein-protein interactions remain a significant development area. The all-vs.-all sequencing (AVA-Seq) method utilizes a convergent fusion plasmid design to make two-hybrid technology amenable to next-generation sequencing. Here, we further innovate to take advantage of synthetic DNA technologies and Oxford Nanopore Technologies long-read sequencing improvements to allow us to determine full-length protein-protein interactions. Here, using this approach we recovered 159 protein-protein interactions from a set of 57 human proteins using multiple forms of validation. Further, when referencing a human gold standard set of interactions, eight full-length protein-protein interactions were recovered from an expected 28 interaction pairs (28.6%), a typical recovery rate for two-hybrid technologies. The AVA-Seq, in combination with the ease of synthetic DNA production and the MinION platform, offers a low-cost, high-throughput alternative for determining protein-protein interactions, which can be utilized in research labs at all stages."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"","description":"Long-read sequencing to detect full-length protein-protein interactions","dates":{"last_updated":"2024-04-07","first_public":"2024-04-07"},"accession":"PRJNA1095688","cross_references":{}}