ENAGenomicsMultiomicsImmunology, Garvan Institute of Medical Researchhttps://www.ebi.ac.uk/ena/browser/view/PRJNA109773Homo sapiensxref:PubMed:19124732Enhanced secondary Ab responses are a vital component of adaptive immunity, yet little is understood about the intrinsic and extrinsic regulators of naïve and memory B cells that results in differences in their responses to Ag. Microarray analysis, together with surface and intracellular phenotyping, revealed that memory B cells have increased expression of members of the TNF receptor, SLAM, B7 and Bcl2 families, as well as the TLR-related molecule CD180 (RP105). Accordingly, memory B cells exhibited enhanced survival, proliferation and Ig secretion, as well as entered division more rapidly than naïve B cells in response to both T-dependent and T-independent stimuli. Furthermore, both IgM and isotype switched memory B cells, but not naïve B cells, co-stimulated CD4+ T cells in vitro through a mechanism dependent on their constitutive expression of CD80 and CD86. This study demonstrates that upregulation of genes involved in activation, co-stimulation and survival provides memory B cells with a unique ability to produce enhanced immune responses and contributes to the maintenance of the memory B cell pool. Keywords: cell type comparison Overall design: Four subsets of human splenic B cells (naïve, IgM-memory, Ig isotype switched memory and plasma cells) sort-purified and analysed immediately ex vivo performed in duplicate.ENAHuman, B-cell, B Cells, human being, Gene Expressions, Man (Taxonomy), B lymphocyte, Homo sapiens, B-Lymphocyte, B Lymphocytes, Modern Man, Modern, B cell, Gene, Expression, Bursa-Equivalent Lymphocyte, B-lymphocyte, Expressions, man, Man, human, Bursa-Dependent Lymphocytes.Human, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManfalseHomo sapiensGene expression by human splenic B-cell subsets2025-09-242014-02-11PRJNA109773GSE13411960619124732