ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR286/029/SRR28621929/SRR28621929.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR286/031/SRR28621931/SRR28621931.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR286/030/SRR28621930/SRR28621930.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR286/028/SRR28621928/SRR28621928.fastq.gzprimaryOK200GenomicsSkin lab, Clinical Insititute of immunology, Shanghai general hospitalhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1098861Mus musculusDuring the activation of CD4+T cells, the pri-miRNAs translocated from cytoplasma into nucleus.However, the mechanism remains unkown. We found that pri-miR-31 interact with nucleolin through G-guadruplex struction in pri-miR-31. We then transfected ASO to destribute the interaction of nucleolin and G4 struction. miRNA-seq was performed in our study. Overall design: ASO and its control CRO was transfected into naïve CD4+ T cells. The transfected cells were activated by anti-cd3/28. After 48 hours, cells were collected and went for RNA extraction. miRNA-seq was then ferformed.ENAfalsemiRNA-seq of CD4+ T cells transfected by ASO or CROmiRNA-seq of CD4+ T cells transfected by ASO or CRO2025-09-242025-04-12PRJNA1098861GSE26373010090