ENAGenomicsPeking Union Medical College Hospitalhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1099380Frostbite is the most common cold injury and is caused by both immediate cold-induced cell death and the gradual development of localized inflammation and tissue ischemia. Delayed healing of frostbite often leads to scar formation, which not only causes psychological distress but also tends to result in the development of secondary malignant tumors. Therefore, a rapid healing method for frostbite wounds is urgently needed. Herein, we used a mouse skin model of frostbite injury to evaluate the recovery process after frostbite. Moreover, single-cell transcriptomics was used to determine the pattern of changes in monocytes, macrophages, epidermal cells and fibroblasts during frostbite. Most importantly, human-induced pluripotent stem cell-derived skin organoids were constructed for the treatment of frostbite. The results showed that skin organoid treatment significantly accelerated wound healing by reducing early inflammation after frostbite and increasing the number of epidermal stem cells. Moreover, in the later stage of wound healing, organoids reduced the number of fibroblasts and regulated ECM expression. These findings indirectly and directly reveal the potential effect of skin organoids in inhibiting scar formation. These results highlight the potential application of organoids for promoting the reversal of frostbite-related changes and the recovery of skin functions. This study provides a new therapeutic alternative for patients suffering from disfigurement and skin dysfnction caused by frostbite.ENAfalseApplication of iPSC-derived skin organoids for frostbite treatment2024-05-112024-05-11PRJNA1099380