ENAapplication/xmlprimary200OKM19736GenomicsAntibiotic Resistance Mechanisms Research Laboratoryhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1102182Escherichia colipathogen:prioritypathogen:bacteriumxref:EuropePMC:PMC11089171pathogenIn the present study, we wonder about the ability of E. coli M19736 clinical strain belonging to sporadic clone ST615 that was one of the first isolates harboring the mcr-1 gene in Latin America , to adapt to XDR phenotype by conjugation and transformation assays. The conservation along time of ARG in evolved MDR and XDR-E. coli M19736 strains showed different patterns of maintenance as well as variability in the capacity to transfer in turn the acquired conjugative plasmids including co-transfer of different plasmids. In addition, we identified EV in the native and in the evolved XDR-E. coli M19736 strains with crucial content for the competence of Antimicrobial resistance in the nosocomial niche, including the blaCTX-M-15 gene though the pDCAG1-CTX-M-15 was previously lost. As a whole, this scenario reveals the relevant role of sporadic clones as common vectors for the dissemination of acquired antimicrobial resistance within the framework of the HGT processes.bacteriaENAfalseEscherichia coli strain:M19736Escherichia coli strain:M19736 Genome sequencing2025-09-242024-04-23PRJNA1102182562