ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/020/SRR29127720/SRR29127720_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/022/SRR29127722/SRR29127722_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/025/SRR29127725/SRR29127725_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/024/SRR29127724/SRR29127724_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/023/SRR29127723/SRR29127723_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/021/SRR29127721/SRR29127721_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/023/SRR29127723/SRR29127723_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/020/SRR29127720/SRR29127720_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/025/SRR29127725/SRR29127725_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/024/SRR29127724/SRR29127724_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/022/SRR29127722/SRR29127722_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR291/021/SRR29127721/SRR29127721_1.fastq.gzprimary200OKGenomicsPharmacology, Keio University School of Medicinehttps://www.ebi.ac.uk/ena/browser/view/PRJNA1114685Mus musculusSerine metabolism provides essential metabolites for cellular growth and proliferation, and also produces neurotransmitters. However, how serine metabolism coordinates with functional development of neurons remains unclear. We report neurotransmitter D-serine inhibit growth of immature cells. Metabolomic analysis of neural progenitors revealed that D-serine decreases glycine synthesis thereby diminishes one-carbon metabolism, in which L-serine is a crucial carbon donor. D-serine inhibits one-carbon metabolism by competing transport of cytosolic L-serine to mitochondria, which restrains proliferation and triggers apoptosis of neural progenitors as well as neural tumor cells, but not mature neurons, in vitro and ex vivo. This RNA-seq data supports the idea that D-serine inhibits polarization and growth/proliferation of immature neurons and further indicate that immature neurons counteracts D-serine-induced cellular stress through enhancing mitochondrial function, including energy synthesis. Overall design: Neuro2a cells were cultured in D-MEM with 10% FBS. The medium was changed to D-MEM(-SG) including 10% FBS with or without 5 mM D-serine. Cells were harvested in Trizol after 18 hours of incubation at 37 °C in a CO2 incubator. RNA was extracted from cells according to the manufacture’s protocol. mRNA libraries of each samples were prepared using a TruSeq Stranded mRNA library Kit (Illumina, San Diego, CA) according to the manufactures’ protocol and paired end sequences were read by NovaSeq (Illumina).ENA(2R)-2-amino-3-hydroxypropanoic acid, Cell., Gene, Expression, Gene Expressions, Expressions, (R)-2-Amino-3-hydroxy-propionic acid, DSN, (R)-2-amino-3-hydroxypropanoic acid, D-Serin(2R)-2-amino-3-hydroxypropanoic acid, Cell., Gene, Expression, Gene Expressions, Expressions, (R)-2-Amino-3-hydroxy-propionic acid, DSN, (R)-2-amino-3-hydroxypropanoic acid, D-SerinfalseEffect of D-serine on gene expression in Neuro2a cellsEffect of D-serine on gene expression in Neuro2a cells2025-09-242024-12-02PRJNA1114685GSE26810610090