ENAGenomicssouthwest universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1124349The anaerobic cultivated sludge was used as seed sludge, and common substrates and intermediate metabolites in fermentation were used as single carbon sources to construct a simplified methanogenesis system. Combined with metagenomic sequencing, the simplified methanogenesis system of different single carbon sources were studied from the overall fermentation performance to the structure and function of microbial community.ENAfalseThe function of simplified methanogenesis system2025-07-182025-07-18PRJNA1124349