{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR296/028/SRR29695428/SRR29695428_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR296/027/SRR29695427/SRR29695427_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR296/026/SRR29695426/SRR29695426_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR296/031/SRR29695431/SRR29695431_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR296/030/SRR29695430/SRR29695430_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR296/026/SRR29695426/SRR29695426_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR296/028/SRR29695428/SRR29695428_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR296/027/SRR29695427/SRR29695427_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR296/031/SRR29695431/SRR29695431_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR296/030/SRR29695430/SRR29695430_2.fastq.gz"],"Other":["ftp://"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Fujian Medical University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA1131099"],"long_description":["Inhibition of the assembly of the RBPJ transcription complex is known to alleviate T cell exhaustion by suppressing PDL1 expression. However, the role of RBPJ in T cells, especially whether inhibition of the assembly of the RBPJ transcription complex is involved in the anti-tumor activity of T cells, remains largely unknown. In this study, we used co-immunoprecipitation to identify HUWE1 as the E3 ubiquitin ligase for RBPJ combined application of CUT&amp Tag, ChIP/ATAC-qPCR, co-immunoprecipitation and flow cytometry indicated that RBPJ led to T cell dysfunction by reshaping the epigenetic landscape, enhancing the transcriptional activity or histone methylation/acetylation of exhausted genes finally, virtual screening and molecular dynamics simulation were used to find that Acarbose reshaped the assembly of the RBPJ-RUVBL1/DNA ternary complex to disrupt the function of RBPJ. Our results not only revealed the mechanism by which RBPJ expression was induced in exhausted T cells, but also explained the molecular mechanism of how RBPJ exacerbated T cell exhaustion. Furthermore, the molecular conformational shaping of the RBPJ transcriptional complex by Acarbose provided new insights into the role of targeted inhibition of T cell exhaustion in the treatment of hepatocellular carcinoma."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"","description":"Epigenetic landscape remodeled by the transcription factor RBPJ promotes tumor-infiltrating T cell exhaustion_ScRNA-Seq","dates":{"last_updated":"2024-07-09","first_public":"2024-07-09"},"accession":"PRJNA1131099","cross_references":{}}