ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/098/SRR29763198/SRR29763198_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/002/SRR29763202/SRR29763202_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/096/SRR29763196/SRR29763196_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/003/SRR29763203/SRR29763203_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/099/SRR29763199/SRR29763199_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/006/SRR29763206/SRR29763206_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/007/SRR29763207/SRR29763207_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/004/SRR29763204/SRR29763204_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/002/SRR29763202/SRR29763202_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/007/SRR29763207/SRR29763207_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/099/SRR29763199/SRR29763199_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/095/SRR29763195/SRR29763195_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/001/SRR29763201/SRR29763201_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/008/SRR29763208/SRR29763208_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/005/SRR29763205/SRR29763205_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/004/SRR29763204/SRR29763204_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/001/SRR29763201/SRR29763201_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/008/SRR29763208/SRR29763208_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/000/SRR29763200/SRR29763200_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/097/SRR29763197/SRR29763197_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/003/SRR29763203/SRR29763203_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/096/SRR29763196/SRR29763196_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/097/SRR29763197/SRR29763197_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/006/SRR29763206/SRR29763206_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/005/SRR29763205/SRR29763205_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/000/SRR29763200/SRR29763200_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/095/SRR29763195/SRR29763195_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR297/098/SRR29763198/SRR29763198_2.fastq.gzprimaryOK200GenomicsPeking Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1134114Homo sapiensDespite the acknowledged effectiveness of BCMA chimeric antigen receptor T (CAR-T) cells in killing multiple myeloma (MM) cells, predicting treatment responsivity to BCMA CAR-T therapy remains a challenge. In this study, we demonstrated that the best overall responses (BORs) of patients over the 15-month follow-up are positively correlated with the abundance and targeted cytotoxic activity of CD8+ effector CAR-T cells on day 28 after CAR-T cell infusion. Additionally, favorable responses are associated with attenuated immunosuppression mediated by regulatory T cells (Tregs), enhanced CD8+ effector T cell cytotoxic activity, and elevated type 1 conventional dendritic cell (cDC1) antigen presentation ability. Our study sheds light on MM microenvironment dynamics after BCMA CAR-T therapy, offering clues for predicting treatment responsivity. Overall design: The collected paired bone marrow (BM) specimens from MM patients before and after BCMA CAR-T therapy were isolated into single cell suspensions, and 3'-scRNA-seq (Chromium Single Cell 3' v3 Libraries) analysis was performed on each sample.ENAfalseReconstitution of the Multiple Myeloma Microenvironment Following Lymphodepletion with BCMA CAR-T TherapyReconstitution of the Multiple Myeloma Microenvironment Following Lymphodepletion with BCMA CAR-T Therapy2025-09-242024-07-14PRJNA1134114GSE2719159606