ENAGenomicsGladuate School of Agricultural Science, Tohoku Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA114877Oryza sativaRice grown in paddy fields prefers to use ammonium ions as a major source of inorganic nitrogen. Glutamine synthetase (GS) catalyzes the conversion of ammonium ions to glutamine. In three cytosolic GS in rice, OsGS1 1 has the critical role for normal growth and grain filling. To understand a role of GS1 1, we performed transcriptional profiling of wild type Nipponbare and GS1 1 mutant plants in seedling using the Agilent Rice Oligo Microarray. Overall design: organisms: Oryza sativa L. cv. Nipponbare mutant: GS1 1 mutant rice (Tos17 insertion NC2373) growth substrates: The germinated seedlings were grown hydroponically in one-fourth concentration of the hydroponic culture solution with 1.0 mM NH4Cl as nitrogen source. The culture solution was replaced as new one every morning at 10:00 am. age: 18 days after sowing tissue: 4th leaf blade derived from 4 plants tissue: 4th leaf sheath derived from 4 plants tissue: roots derived from a plant WT vs. GS1 1 mutant rice hybridization : two dye method hybridization: Dye-swap experimentENAL-Glutamate:ammonia ligase (ADP-forming), Ligase, Rice., glutamine synthase clone F11, F15E12_14, red rice <Oryza sativa>, Glutamate Ammonia Ligase, Gene Expressions, rice, Glutamate-Ammonia, Rices, Glutamate Ammonia Ligase (ADP), red rice, Synthetase, Oryza sativa, ATGSR2, Gene, Expression, F15E12.14, GLUTAMINE SYNTHETASE, Expressions, Glutamine Synthetase, Asian cultivated rice, Glutaminered rice, Oryza sativa, Rice., red rice <Oryza sativa>, rice, Asian cultivated rice, RicesfalseOryza sativaGene expression of cytosolic glutamine synthetase 1 1 knock-out mutant rice2025-09-242014-02-11PRJNA114877GSE151224530