ENAGenomicsRIKENhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1151630Prolonged high-temperature stress affects plant growth and development, posing a major threat to ecological diversity and agricultural productivity. We investigated the effects of prolonged high-temperature stress on Brachypodium distachyon, revealing iron deficiency in newly emerged leaves. Application of proline-2'-deoxymugineic acid (PDMA), which is a synthetic phytosiderophore analog, enhanced resilience in Brachypodium Bd21 under high temperatures. This study that a chelation-based iron uptake strategy in conferring resilience to prolonged high-temperature stress in temperate grasses offers new insights into the development of crop management strategies aimed at enhancing crop resilience in the warming climate.ENAfalseEffect of PDMA treatment under high-temperature conditions in Brachypodium Bd212025-07-282025-07-28PRJNA1151630