{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics","Multiomics"],"center_name":["HHMI"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA115489"],"scientific_name":["Mus musculus"],"long_description":["Journal : Blood. 2009 Jul 9 114(2):469-77. Epub 2009 May 13. Title : Endothelial deletion of hypoxia-inducible factor-2alpha (HIF-2alpha) alters vascular function and tumor angiogenesis. Authors : Skuli N, Liu L, Runge A, Wang T, Yuan L, Patel S, Iruela-Arispe L, Simon MC, Keith B. Abstract : Hypoxia-inducible factor-2alpha (HIF-2alpha) is highly expressed in embryonic vascular endothelial cells (ECs) and activates the expression of target genes whose products modulate vascular function and angiogenesis. In this report, we describe a genetic model designed to test the physiologic consequences of deleting HIF-2alpha in murine endothelial cells. Surprisingly, mice with HIF-2alpha-deficient ECs developed normally but displayed a variety of phenotypes, including increased vessel permeability, aberrant endothelial cell ultrastructure, and pulmonary hypertension. Moreover, these animals exhibited defective tumor angiogenesis associated with increased hypoxic stress and tumor cell apoptosis. Immortalized HIF-2alpha-deficient ECs displayed decreased adhesion to extracellular matrix proteins and expressed reduced levels of transcripts encoding fibronectin, integrins, endothelin B receptor, angiopoietin 2, and delta-like ligand 4 (Dll4). Together, these data identify unique cell-autonomous functions for HIF-2alpha in vascular endothelial cells. Keywords: Murine lung endothelial cell study Overall design: Cnt1,2 and 3 (Hif-2a floxed/floxed) cells were subjected to 0.5% oxygen treatment for 16hrs and KO1,2 and 3 (Hif-2a knockout) cells were subjected to 0.5% oxygen treatment for 16hrs."],"tag":["xref:PubMed:19439736"],"repository":["ENA"],"description_synonyms":["Endothelial Cells, alpha-Ada, anon-EST:fe2E2, BamF, l(3)alpha-Spec, determination, BamC, AP-2, alpha-Spect, Deficiencies, alpha-Spe, Gene, Capillary, Hypoxia., CG31654, alpha-Spc, l(2)SH2 0460, prevention, Vascular Endothelial, fs(3)neo61, Oxygen Deficiency, Oxygen Deficiencies, CG10422, alpha-ada, l(3)04276, Lymphatic, parenchyma of lung, Vascular, klo, lung, alpha-adaptin, alpha-Sp, Lymphatic Endothelial Cell, DmelCG10422, CG1977, prevention and control, neoplasm, alpha-spec, Vascular Endothelial Cells, Gene Expressions, reference sample, CG4260, Vascular Endothelial Cell, dre3, Alpha-Adaptin, Expressions, Lymphatic Endothelial Cells, alpha-spectrin, preventive measures, Oxygen, Capillary Endothelial, Expression, Capillary Endothelial Cell, Controlled, l(3)dre3, Controlling, preventive therapy, alpha-Spectrin, pulmo, Capillary Endothelial Cells, 3A9, DmelCG1977, lung parenchyma, ham, Cell, ada, Anoxia, alphaSpec, D-Ada, chemical analysis, cg4260, MENE(2L)-A, dAP-2a, alphaSp, spectrin, neoplasms, l(2)06694, MENE (2L)-A, D-alphaAda, mating_type_alpha, prophylaxis, FCP-B, Hypoxemia, alpha, AP-2alpha, Endothelial, DmelCG4260, AP2, Lymphatic Endothelial, Deficiency, pulmonary, control, l(3)62Bd, Bam-C, Endothelial Cell, BAM, Cells, Bam, alpha mating type (yeast), l(2)SH0460, alfa-Spec, Lungs, assay, Anoxemia, Spec"],"name_synonyms":["Mus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscaris"],"additional_accession":[]},"is_claimable":false,"name":"Mus musculus","description":"Gene expression analysis in control and HIF-2 alpha deficient murine lung endothelial cells under hypoxia","dates":{"last_updated":"2025-09-24","first_public":"2014-02-11"},"accession":"PRJNA115489","cross_references":{"GEO":["GSE16067"],"taxon":["10090"],"PubMed":["19439736"]}}