{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Functional Genomics Center Zurich (FGCZ), ETH ZURICH / University of Zurich"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA1160222"],"scientific_name":["Mus musculus"],"long_description":["Hepatocellular carcinoma (HCC) mostly develops on the background of a chronic liver disease (CLD). The hallmarks of CLD includes increased hepatocyte apoptosis, compensatory proliferation and DNA damage. Mice with MCL-1 deficiency in hepatocytes (Mcl-1Dhep mice ) recapitulated these key features of CLD and developed HCC later in life. However, it is unclear how MCL-1 deficiency drives tumorigenesis. We analysed replication stress and the activation the cGAS-STING pathway in Mcl-1Dhep mice. 2-month-old Mcl-1Dhep mice showed increased replication stress, micronucleated hepatocytes and increased cytosolic DNA sensing. We found that an increase in extrachromosomal circular DNA in Mcl-1Dhep liver promoted a crosstalk between hepatocytes and immune cells to drive the immune response which was dependent on the canonical cGAS-STING pathway. We then generated Mcl-1Dhep/STING-/- double knockout mice to study the effects of inhibiting the cGAS-STING pathway in liver homeostasis and tumorigenesis. In fact, deletion of STING in Mcl-1Dhep mice reduced immune cell chemotaxis, as well as tumor incidence. Our findings indicate that the cytosolic DNA sensing pathway may play an important role in inflammation-driven liver carcinogenesis. Overall design: Knockout mice were generated by crossing transgenic mice with different genetic background."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"The effect of inhibiting the cGAS-STING pathway in a chronic liver disease mouse model","description":"The effect of inhibiting the cGAS-STING pathway in a chronic liver disease mouse model","dates":{"last_updated":"2025-09-24","first_public":"2025-09-13"},"accession":"PRJNA1160222","cross_references":{"GEO":["GSE277012"],"taxon":["10090"]}}