{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["University of Toronto"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA1167332"],"scientific_name":["Homo sapiens"],"tag":["xref:PubMed:40604231"],"long_description":["Pancreatic ductal adenocarcinoma is a high-mortality cancer with an aggressive, treatment resistant phenotype and a complex tumour microenvironment featuring significant hypoxia. We leveraged a unique 3D in vitro platform (TRACER) to perform single-cell transcriptome analysis of organoids cultured in a spatially-defined microenvironmental gradient to explore the effect of oxygen and other microenvironmental gradients on organoid heterogeneity. Overall design: Pancreatic cancer patient-derived organoids (PDOs) were dissociated to single cells and cultured in the TRACER platform. After 24h of culture in the rolled configuration featuring microenvironmental gradients, cells from each of the 6 layers were isolated from the construct and analyzed by scRNA-seq."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Single cell analysis of an engineered organoid based model of pancreatic cancer identifies hypoxia as a contributing factor in the determination of transcriptional subtypes","description":"Single cell analysis of an engineered organoid based model of pancreatic cancer identifies hypoxia as a contributing factor in the determination of transcriptional subtypes","dates":{"last_updated":"2025-09-24","first_public":"2025-07-17"},"accession":"PRJNA1167332","cross_references":{"GEO":["GSE278477"],"taxon":["9606"],"PubMed":["40604231"]}}