ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/003/SRR31143303/SRR31143303_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/097/SRR31143297/SRR31143297_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/099/SRR31143299/SRR31143299_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/005/SRR31143305/SRR31143305_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/007/SRR31143307/SRR31143307_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/001/SRR31143301/SRR31143301_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/000/SRR31143300/SRR31143300_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/004/SRR31143304/SRR31143304_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/096/SRR31143296/SRR31143296_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/001/SRR31143301/SRR31143301_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/007/SRR31143307/SRR31143307_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/099/SRR31143299/SRR31143299_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/006/SRR31143306/SRR31143306_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/002/SRR31143302/SRR31143302_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/098/SRR31143298/SRR31143298_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/000/SRR31143300/SRR31143300_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/096/SRR31143296/SRR31143296_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/004/SRR31143304/SRR31143304_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/006/SRR31143306/SRR31143306_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/003/SRR31143303/SRR31143303_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/097/SRR31143297/SRR31143297_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/098/SRR31143298/SRR31143298_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/002/SRR31143302/SRR31143302_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR311/005/SRR31143305/SRR31143305_2.fastq.gzprimaryOK200GenomicsChinese Academy of Scienceshttps://www.ebi.ac.uk/ena/browser/view/PRJNA1178728Osmotic stress triggers Ser2-phosphorylated RNA polymerase II (RNAPII Ser-2P) ubiquitylation and degradation, mediated by Cullin E3 ligase complexes where activated CUL1-FBXO11 complex showed prominent role. Pan-Cullin E3 ligase inhibitor MLN4924 treatment totally reversed osmotic stress induced RNAPII degradation, consequently, osmotic stressed 293T cells (120mM Kcl for 2 hours), who are pretreated with 0.01mM MLN4924 for 2 hours, were utilized to perform RNAPII Ser-2P specific CUT&amp Tag assay. NT (PBS, DMSO), Kcl (120mM Kcl, DMSO) and MLN4924 (PBS, 0.01mM MLN4924) groups were all conducted as controls, and each group possesses 3 biological replicates. Dissecting genome wide RNAPII Ser-2P binding offers the landscape of osmotic stress induced transcription changes and RNAPII pool size alteration effects on global transcription.ENAfalseRNAPII Ser-2P CUT&Tag assay following osmotic stress and MLN4924 treatment2024-10-302024-10-30PRJNA1178728