ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR313/072/SRR31353072/SRR31353072_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR313/072/SRR31353072/SRR31353072_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR313/073/SRR31353073/SRR31353073_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR313/073/SRR31353073/SRR31353073_1.fastq.gzprimary200OKGenomicsNational University of Singaporehttps://www.ebi.ac.uk/ena/browser/view/PRJNA1186198Mus musculusWhilst M. bovis Bacillus Calmette-Guérin (BCG) therapy remains the gold-standard for treatment of high-risk non-muscle invasive bladder cancer (BC), 30-40% of patients fail therapy, resulting in disease recurrence and progression. Loss of glutathione-S-transferase theta 2 (GSTT2) expression has been associated with improved response to fewer instillations of BCG. To understand these responses, wild-type (WT) and GSTT2-knockout (KO) mice were implanted orthotopically with MB49-PSA BC cells, and mice were treated with four weekly BCG instillations, after which the bladders were harvested for single-cell RNA sequencing. Overall design: Single cells, isolated from the bladder of BCG-treated WT and KO mice, were barcoded using the whole-transcriptome kit from Parse Biosciences and sequenced (HiSeq PE150). The reads were aligned to the mouse genome (GRCm39) with the split-pipe (v0.9.6p) program (from Parse).xref:PubMed:39769061ENAfalseGlutathione-S-transferase theta 2 (GSTT2) modulates the tumor microenvironment and the response to BCG immunotherapy in a murine orthotopic model of bladder cancerGlutathione-S-transferase theta 2 (GSTT2) modulates the tumor microenvironment and the response to BCG immunotherapy in a murine orthotopic model of bladder cancer2025-09-242024-12-12PRJNA1186198GSE2819321009039769061