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k/vol1/fastq/SRR323/000/SRR32302900/SRR32302900_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR323/004/SRR32302904/SRR32302904_2.fastq.gzprimaryOK200GenomicsDepartment of Medicinal and Life Sciences, Tokyo University of Sciencehttps://www.ebi.ac.uk/ena/browser/view/PRJNA1221959Mus musculusCaloric restriction (CR) is a well-established intervention that extends lifespan and delays the onset of age-related diseases in various species. While previous studies have demonstrated CR-induced transcriptional changes in white adipose tissue (WAT), the impact of CR on alternative splicing remains underexplored. In this study, we performed RNA sequencing (RNA-seq) to investigate gene expression and alternative splicing in subcutaneous white adipose tissue (sWAT) of wild-type mice subjected to CR for six months, from 3 to 9 months of age. RNA-seq analysis identified 6,058 differentially expressed genes (DEGs) (p 1.0) between ad libitum (AL) and CR conditions, with key upregulated genes related to lipid metabolism, including Elovl6, Fasn, Acc, and Srebp1c. Additionally, we detected 400 significant alternative splicing events (FDR < 0.05), with exon skipping being the most prevalent. Among these, we focused on the metabolism-related transcription factor Ncoa2, which exhibited CR-induced exon 13 inclusion, shifting the balance toward the full-length isoform. Functional analyses suggest that this shift enhances Ncoa2 coactivator activity for nuclear receptors involved in lipid metabolism. This dataset provides raw and processed RNA-seq data, including gene expression and alternative splicing analyses, which can be utilized to further explore the molecular mechanisms underlying CR-induced metabolic adaptations in adipose tissue. Overall design: RNA-seq analysis of subcutaneous white adipose tissue (sWAT) from wild-type C57BL/6J mice subjected to calorie restriction (CR) for six months (3 to 9 months of age). This study aims to investigate the effects of CR on gene expression and alternative splicing, with a focus on lipid metabolism and transcriptional regulators such as Ncoa2. Four biological replicates were analyzed for each condition (ad libitum [AL] and calorie restriction [CR]). Samples were collected at 9 months of age. PolyA-enriched RNA libraries were prepared and sequenced using paired-end reads on the Illumina NextSeq 500 platform, generating approximately 400 million reads per sample.xref:PubMed:40267838ENAfalseRNA-seq analysis of subcutaneous white adipose tissue from wild-type mice subjected to six months of calorie restriction reveals altered NCOA2 splicing.RNA-seq analysis of subcutaneous white adipose tissue from wild-type mice subjected to six months of calorie restriction reveals altered NCOA2 splicing.2025-09-242025-04-23PRJNA1221959GSE2892401009040267838