{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Shanghai Institute of Materia Medica"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA1230784"],"long_description":["We report that IL22, a cytokine highly upregulated after partial hepatectomy or hepatocyte transplantation, could support long-term expansion (>30 passages, with theoretical expansion of ~1025 times within ~150 days) of mice hepatocytes in vitro by dedifferentiate hepatocytes into hepatocyte progenitor cells (HPCs), which maintain the capacity of differentiation into mature hepatocytes. With transcriptomic analysis and lineage-specific deletion, we uncover the critical involvement of STAT3 pathway in IL22-mediated hepatocyte to HPC conversion. Two key transcription factors (TFs) down-stream of STAT3 pathway, Bhlha15 and Arntl2, govern IL22-induced hepatic dedifferentiation. Expression of these two TFs directly initiates the dedifferentiation of hepatocytes into HPCs, which could be expanded in long-term without the supplement of IL22. IL22 also supports human hepatocyte growth in simple culture condition. Within 30 days, human hepatocytes could be expanded by more than 10,000-fold by dedifferentiation into HPCs, which maintain the full capacity of maturation."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"","description":"RNA-seq transcriptional profiling in IL22 induced mouse and human hepatocytes in vitro, IL22 induced WT and STAT3 knock-out hepatocytes in vitro, Bhlha15 and Arntl2 induced hepatocytes in vitro.","dates":{"last_updated":"2025-03-12","first_public":"2025-03-12"},"accession":"PRJNA1230784","cross_references":{}}