{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR325/040/SRR32556340/SRR32556340_subreads.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR325/038/SRR32556338/SRR32556338_subreads.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR325/039/SRR32556339/SRR32556339_subreads.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR325/041/SRR32556341/SRR32556341_subreads.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR325/037/SRR32556337/SRR32556337_subreads.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Broad Institute of MIT and Harvard"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA1231058"],"long_description":["Double-strand break (DSB) repair is highly mutagenic compared to normal replication. In budding yeast, repair of an HO endonuclease-induced DSB at MAT-alpha can be made with a transcriptionally silent HMR::Kl-URA3 donor. We isolated thousands of mutations occurring during this repair process. We conclude that the repair replication fork appears to have the two DNA strands open ~80 bp ahead of the DNA polymerase, but the strands re-anneal rapidly behind the polymerase. Additionally, we analyzed interchromosomal template switching, in which the partially copied DNA strand dissociates and pairs with a new template at a short stretch of perfectly matching bases (microhomology), and resumes copying. We show that these apparent microhomology-mediated template switching events in fact require the pairing of ~200 bp of imperfectly matching bases (homeology)."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"","description":"Mutations and structural variants arising during double-strand break repair","dates":{"last_updated":"2025-03-05","first_public":"2025-03-05"},"accession":"PRJNA1231058","cross_references":{}}