ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/014/SRR32843614/SRR32843614_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/006/SRR32843606/SRR32843606_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/010/SRR32843610/SRR32843610_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/009/SRR32843609/SRR32843609_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/013/SRR32843613/SRR32843613_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/004/SRR32843604/SRR32843604_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/002/SRR32843602/SRR32843602_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/016/SRR32843616/SRR32843616_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/013/SRR32843613/SRR32843613_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/007/SRR32843607/SRR32843607_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/005/SRR32843605/SRR32843605_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/003/SRR32843603/SRR32843603_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/012/SRR32843612/SRR32843612_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/006/SRR32843606/SRR32843606_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/015/SRR32843615/SRR32843615_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/012/SRR32843612/SRR32843612_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/008/SRR32843608/SRR32843608_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/016/SRR32843616/SRR32843616_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/004/SRR32843604/SRR32843604_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/007/SRR32843607/SRR32843607_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/010/SRR32843610/SRR32843610_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/011/SRR32843611/SRR32843611_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/015/SRR32843615/SRR32843615_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/003/SRR32843603/SRR32843603_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/009/SRR32843609/SRR32843609_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/014/SRR32843614/SRR32843614_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/011/SRR32843611/SRR32843611_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/002/SRR32843602/SRR32843602_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/008/SRR32843608/SRR32843608_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR328/005/SRR32843605/SRR32843605_1.fastq.gzprimary200OKGenomicsFudan Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1241486This project aims to investigate whether glycan modifications influence the transcriptome of HCT116 cells, a human colorectal carcinoma cell line with a fucosylation deficiency caused by a mutation in GDP-mannose-4,6-dehydratase (GMDS). Using glycan modifiers such as fucose, 3-fluorinated sialic acid (3-F-Sia), and Kifunensine (Kif), we generated five distinct glycan phenotypes: sialylated (S), Underacted(Underc), fucosylated (F), sialofucosylated (FS), and high mannose (HM). mRNA sequencing will be performed to explore whether these glycan modifications induce significant transcriptomic changesENAfalsemRNA Sequencing of HCT116 Cells Under Glycan Modifications (Fucose, 3-F-Sia, and Kifunensine2025-03-292025-03-29PRJNA1241486