ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/084/SRR32939084/SRR32939084_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/080/SRR32939080/SRR32939080_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/082/SRR32939082/SRR32939082_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/084/SRR32939084/SRR32939084_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/083/SRR32939083/SRR32939083_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/086/SRR32939086/SRR32939086_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/081/SRR32939081/SRR32939081_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/081/SRR32939081/SRR32939081_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/085/SRR32939085/SRR32939085_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/079/SRR32939079/SRR32939079_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/079/SRR32939079/SRR32939079_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/083/SRR32939083/SRR32939083_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/082/SRR32939082/SRR32939082_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/080/SRR32939080/SRR32939080_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/085/SRR32939085/SRR32939085_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR329/086/SRR32939086/SRR32939086_2.fastq.gzprimaryOK200GenomicsDepartment of Medical Genetics, Ajou University School of Medicinehttps://www.ebi.ac.uk/ena/browser/view/PRJNA1244642Mus musculusActin dynamics, mediated by various actin-binding proteins, plays an important role in adipocyte differentiation. We investigated the role of coactosin-like F-actin binding protein (Cotl1) in adipocyte differentiation in vitro and in vivo. Cotl1 expression level was increased during adipocyte differentiation in mouse 3T3-L1 cells and primary cultured adipose-derived stem cells (ADSCs) and during weight gain in adipose tissues. However, Cotl1 deficient in 3T3-L1 and ADSCs inhibited adipocyte differentiation, and Cotl1-/- mice displayed resistance to high-fat diet (HFD)-induced weight gain, hepatic steatosis and adipocyte enlargement compared to HFD-fed wild type (WT) mice. Ingenuity Pathway Analysis of RNA-sequencing in adipose tissues of HFD-WT and HFD-Cotl1-/- mice predicted complicated relationships between Cotl1, differentiation of adipocytes, obesity and organization of actin cytoskeleton. Particularly, peroxisome proliferator-activated receptor gamma (Pparg) emerged as a central player, with Cotl1 influencing Pparg expression, consequently regulating adipocyte differentiation. These findings suggest Cotl1 as a pivotal regulator of terminal adipocyte differentiation by modulating adipogenic genes. Overall design: Wild-type and Cotl1 knockout mice were fed a high-fat diet for 12 weeks, and adipose tissues were isolated for RNA-Seq analysis.ENAfalseCoactosin-like F-actin Binding Protein (Cotl1) Plays a Key Role in Adipocyte Differentiation and ObesityCoactosin-like F-actin Binding Protein (Cotl1) Plays a Key Role in Adipocyte Differentiation and Obesity2025-09-242025-04-12PRJNA1244642GSE29344510090