ENAGenomicsAlbert Einstein college of Medicinehttps://www.ebi.ac.uk/ena/browser/view/PRJNA1246567Mycobacterium tuberculosis (Mtb) uses a specialized secretion system, ESX-5, to export numerous proteins during infection. Here, we found that deleting ESX-5 from Mtb not only prevented secretion of many ESX-5 substrates but also impacted other ESX systems. The M.tb esx5 mutant had increased ESX-1 substrate secretion but reduced ESX-3 expression.ENAfalseAn ESX-5 full locus deletion in Mycobacterium tuberculosis alters mycomembrane composition and boosts ESX-1 substrate secretion2025-07-042025-07-04PRJNA1246567