ENAGenomicsPharmacology and Chemical Biology, University of Pittsburghhttps://www.ebi.ac.uk/ena/browser/view/PRJNA125013Mus musculusxref:PubMed:33564620The hepatocyte growth factor (HGF)/c-Met signaling pathway is known to mediate vascularization. We have previously demonstrated that expression of a human HGF transgene in the small airways produced mice (HGF TG) that were more susceptible to the tobacco carcinogen, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). We also have observed that HGF TG mice display significantly enhanced vascularization in the lungs that increases over time compared to wild-type (WT) littermates. To analyze which genes might contribute to increased vascularization from HGF overexpression in the airways, RNA and protein were isolated from whole lungs of individual HGF TG and WT adult mice. We profiled the mRNA expression of several hundred genes representative of six biological pathways involved in transformation, angiogenesis, and tumorigenesis using two commercial microarrays. Significant changes in expression over a 1.5-fold boundary were also observed in lung tumors derived from NNK-treated HGF TG mice. Overall design: Lung tumors were induced by exposing mice to four weekly i.p. injections of 3mg NNK (15μg/μl) over 2 weeks. Whole lungs from control untreated animals were dissected after sacrifice at 10, 20, or 40 wks of age, and NNK induced lung tumors were dissected from the animals at 20 or 40 weeks of age. Total RNA was extracted from whole lung or isolated tumors from HGF TG or WT mice using TRIzol reagent and the Array Grade Total RNA Isolation Kit. The cDNA was generated and labeled using the TrueLabeling-AMP Linear RNA Amplication Kit. RNA was analyzed from 28 mice in total. The angiogenesis array was used to analyze 10 samples taken from 40 week old mice (HGF TG untreated [n=4], WT untreated [n=4], HGF TG NNK treated [n=1], WT NNK treated [n=1]) and 6 samples from 20 week old mice (HGF TG untreated [n=2], WT untreated [n=2], HGF TG NNK treated [n=1], WT NNK treated [n=1]). The cancer gene array was used to analyze 8 samples taken from 40 week old mice (HGF TG untreated [n=2], WT untreated [n=2], HGF TG NNK treated [n=2], WT NNK treated [n=2]) and 4 samples from 20 week old mice (HGF TG untreated [n=2], WT untreated [n=2]).ENAGINGF, 3.4.22.-, RNA, Ribonucleic, Initiators, ribose nucleic acid, Laboratory, NS4, Mus domesticus, ribonucleic acids, CASP14, mouse, DFNB39, NK2, NK1, mini-ICE, Initiator, RNS, CASP-14, lungs, Tumor, HSF, House Mouse, BSF2, HGF, C230052L06Rik, GGF1, HGFR, House, Mus, yeast nucleic acid, Ribonukleinsaeure, Oncogen, IL-6, Mini-ICE, Promoters, pulmones, HGF|SF, Gene Products, Mus musculus domesticus, pentosenucleic acids, lungs pair, GF1, Tumor Initiator, Ribonucleic acids, carcinogenic agent, Mice, Tumor Promoter, Non Polyadenylated, RNA Gene Products, F-TCF, ribonucleic acid, Acid, Mus musculus, Tumor., SF|HGF, Caspase-14 subunit p10, Swiss, mice, Non Polyadenylated RNA, Promoter, Swiss Mouse, Tumor Promoters, Non-Polyadenylated, SF, HPTA, House Mice, Swiss Mice, Caspase-14 subunit p19, Ribonucleic Acid, set of lungs, HGFB, c-Met, MICE, HGF/SF, Laboratory Mice, domesticus, Non-Polyadenylated RNA, Tumor Initiators, IFNB2, Oncogens, Human Gingival Fibroblasts, Carcinogen, TG, Lungs, Mouse, Par4, AI838057, SF/HGF, Laboratory MouseMus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscarisfalseMus musculusRNA profiling in lungs of WT and HGF transgenic mice with or without exposure to carcinogen2025-09-242014-02-11PRJNA125013GSE204681009033564620