{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Alzheimer's and Parkinson's Disease laboratory, Medicine, Brain and Mind Research Institute, University of Sydney"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA125103"],"scientific_name":["Mus musculus"],"long_description":["In neurodegenerative disorders such as Alzheimer’s disease (AD), brain miRNA profiles are altered thus miRNA dysfunction could be both a cause and a consequence of disease. Our study dissects the complexity of human AD pathology, in the absence of a post mortem delay, and provides the first miRNA profiling analysis addressing the contribution of amyloid-β (Aβ), a known causative factor of AD, to the de-regulation of neuronal miRNA expression. We used murine primary hippocampal neurons to show that Aβ is a powerful regulator of mature miRNA expression and a prominent miRNA down-regulation is evoked in response to Aβ peptides. Our study provides insight into a previously unexplored mechanism of how Aβ may impact the pathology of AD and identification of key miRNAs affected by Aβ will allow further analysis on target genes and biological pathways contributing to AD pathomechanisms. Overall design: Mouse primary hippocampal neurons were grown for 23 DIV before being treated for 24 hours with either 5uM amyloid beta (1-42) aged for 24 hours at 37 degrees with shaking, or a PBS control. Total RNA was extracted using the Qiagen miRNeasy Kit and expression of 380 microRNA was analysed using rodent TaqMan low density microRNA microarrays (Applied Biosystems)."],"tag":["xref:PubMed:20552018"],"repository":["ENA"],"description_synonyms":["Presenile Alzheimer Dementia, ALZHEIMERS DIS, Alzheimers Diseases, Early Onset, stRNA, Sclerosis, e(-), DELTA AND GAMMA, Amyloid Fibril, electron, betaTub1, Early Onset Alzheimer Disease, Focal Onset, Alzheimer's disease, unspecified (disorder), ALZHEIMER DIS, Primary Senile Degenerative, Small, AD, unspecified, systemic amyloidosis, B1t, [X]Dementia in Alzheimer's disease, B-spec, betaspec, amyloid, amyloidosis, LATE ONSET ALZHEIMER DIS, Small Temporal, Familial Alzheimer Disease (FAD), betatub(56D), Alzheimer Diseases, kus, beta1tub, DAT - Dementia Alzheimer's type, CG9277, Elektron, amyloidosis (disease), beta[[1]] tubulin, beta1t, T, beta-particle, miRNA, Primary miRNA, Focal Onset Alzheimer's Disease, Substance, Amyloid, Late Onset Alzheimer Disease, INSDC_feature:ncRNA, pri-miRNA, FOCAL ONSET ALZHEIMERS DIS, Presenile Dementia, anon-EST:fe1B3, 1t, e, e-, beta1Tub, AD - Alzheimer's disease, SPEC8, Alzheimer Disease, Alzheimer Type Dementia, ALZHEIMER DIS EARLY ONSET, Alzheimer's disease (disorder), CG5870, Presenile, Alzheimers Dementia, pre miRNA, Alzheimers disease, Alzheimer Senile Dementia, beta-tubulin56D, Spec-beta, Senile, RNA, Disease, Dmbeta1, Alzheimer disease, BETA 56D, Alzheimer Syndrome, Acute Confusional Senile Dementia, familial, Small Temporal RNA, Tubulin, Primary, i168, Alzheimer's Dementia, Primary Senile Degenerative Dementia, [X]Dementia in Alzheimer's disease (disorder), Alzheimer Type, Alzheimer Dementias, Amyloid Fibrils, Micro RNA, l(1)G0108, Alzheimer's Diseases, betaSpec, beta1-Tubulin., NOS, FLOWERING TIME CONTROL PROTEIN FCA ALPHA, pre-miRNA, b-Spec, amyloid disease, Dementia, Fibril, pri miRNA, beta-tub, DmelCG5870, miRNAs, l(1)G0074, Alzheimers, Amyloid Substance, amyloidoses, l(1)G0198, Beta <eudicots>, negatron, Micro, Temporal RNA, Dementia in Alzheimer's disease, beta[[1]]-tubulin, Alzheimer, Alzheimer's Disease Pathway, b spectrin, FCAALL.331, Primary MicroRNA, Dementias, Alzheimer Type Senile Dementia, beta1, beta56D, Alzheimer-Type (ATD), DmelCG9277, Alzheimer Dementia, beta, Alzheimer Sclerosis, beta1-tub, beta(-), DTB2, Tub, beta-Tub, Fibrils, Senile Dementia, Late Onset, beta-spec, Familial (FAD), i6, DL4180C, Acute Confusional, Dementia in Alzheimer's disease (disorder), BETA, Alzheimer-Type Dementia (ATD), MicroRNA, Alzheimer's Disease, beta-Tub56D, Familial Alzheimer Diseases (FAD), Spec, betaTub, Specbeta, Alzheimer Type Dementia (ATD), Dementia of the Alzheimer's type"],"name_synonyms":["Mus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscaris"],"additional_accession":[]},"is_claimable":false,"name":"Mus musculus","description":"Neuronal microRNA response to Alzheimer's disease amyloid beta","dates":{"last_updated":"2025-09-24","first_public":"2013-06-27"},"accession":"PRJNA125103","cross_references":{"GEO":["GSE20447"],"taxon":["10090"],"PubMed":["20552018"]}}