ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/069/SRR33242869/SRR33242869_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/065/SRR33242865/SRR33242865_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/070/SRR33242870/SRR33242870_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/058/SRR33242858/SRR33242858_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/057/SRR33242857/SRR33242857_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/066/SRR33242866/SRR33242866_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/063/SRR33242863/SRR33242863_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/056/SRR33242856/SRR33242856_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/058/SRR33242858/SRR33242858_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/068/SRR33242868/SRR33242868_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/059/SRR33242859/SRR33242859_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/071/SRR33242871/SRR33242871_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/070/SRR33242870/SRR33242870_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/061/SRR33242861/SRR33242861_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/065/SRR33242865/SRR33242865_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/064/SRR33242864/SRR33242864_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/062/SRR33242862/SRR33242862_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/067/SRR33242867/SRR33242867_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/060/SRR33242860/SRR33242860_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/063/SRR33242863/SRR33242863_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/064/SRR33242864/SRR33242864_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/056/SRR33242856/SRR33242856_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/071/SRR33242871/SRR33242871_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/069/SRR33242869/SRR33242869_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/062/SRR33242862/SRR33242862_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/057/SRR33242857/SRR33242857_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/059/SRR33242859/SRR33242859_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/068/SRR33242868/SRR33242868_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/066/SRR33242866/SRR33242866_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/067/SRR33242867/SRR33242867_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/060/SRR33242860/SRR33242860_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR332/061/SRR33242861/SRR33242861_2.fastq.gzprimaryOK200GenomicsStanfordhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1253761Mus musculusThe transcription factor TCF-1 is downregulated during effector differentiation of CD8+ T cells. However, how its downregulation impacts gene expression in different effector- and memory-biased populations remains unclear. Here, we generate mice in which TCF-1 fails to become downregulated in differentiated effector cells and probe the transcriptional impact of TCF-1 retention in different T cell differentiation states by scRNA-sequencing. Overall design: CD8+ T cells specific to the LCMV-gp3 antigen were sorted from the spleens of two WT control and two Tcf7∆+22kb mice on 8 dpi with LCMV-Armstrong. Samples were subjected to scRNA-sequencing.ENAfalseEffects of TCF-1 de-repression on heterogeneity of effector and memory progenitor T cell transcriptomes in acute infection [scRNA-seq]Effects of TCF-1 de-repression on heterogeneity of effector and memory progenitor T cell transcriptomes in acute infection [scRNA-seq]2025-09-242025-06-01PRJNA1253761GSE29524610090