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3397091_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR333/094/SRR33397094/SRR33397094_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR333/076/SRR33397076/SRR33397076_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR333/088/SRR33397088/SRR33397088_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR333/082/SRR33397082/SRR33397082_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR333/070/SRR33397070/SRR33397070_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR333/073/SRR33397073/SRR33397073_2.fastq.gzprimaryOK200GenomicsBrangwynne Laboratory, CBE, Princeton Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1257780Homo sapiensBiomolecular condensates are key features of intracellular compartmentalization. As the most prominent nuclear condensate in eukaryotes, the nucleolus is a multiphase liquid-like structure where ribosomal RNAs (rRNAs) are transcribed and processed, undergoing multiple maturation steps to form the small and large ribosomal subunits (SSU and LSU). However, how rRNA processing is coupled to the layered nucleolar organization is poorly understood due to a lack of tools to precisely monitor and perturb nucleolar rRNA processing dynamics. Here, we developed two complementary approaches to spatiotemporally map rRNA processing and engineer de novo nucleoli. Using sequencing in parallel with imaging, we found that rRNA processing steps are spatially segregated, with sequential maturation of rRNA required for its outward movement through nucleolar phases. By generating synthetic nucleoli in cells through an engineered rDNA plasmid system, we show that defects in SSU processing can alter the ordering of nucleolar phases, resulting in inside-out nucleoli and preventing rRNA outflux, while LSU precursors are necessary to build the outermost layer of the nucleolus. These findings demonstrate how rRNA is both a scaffold and substrate for the nucleolus, with rRNA acting as a programmable blueprint for the multiphase architecture that facilitates assembly of an essential molecular machine. Overall design: RNA-seq was performed on 5eU pulse-chase labeled samples to monitor rRNA processing dynamics over time in response to various perturbations affecting ribosome biogenesis.ENAfalseMapping and engineering RNA-driven architecture of the multiphase nucleolusMapping and engineering RNA-driven architecture of the multiphase nucleolus2025-09-242025-06-19PRJNA1257780GSE2960809606