ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/051/SRR33638151/SRR33638151.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/044/SRR33638144/SRR33638144.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/046/SRR33638146/SRR33638146.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/045/SRR33638145/SRR33638145.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/050/SRR33638150/SRR33638150.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/047/SRR33638147/SRR33638147.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/043/SRR33638143/SRR33638143.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/052/SRR33638152/SRR33638152.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/048/SRR33638148/SRR33638148.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/054/SRR33638154/SRR33638154.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/049/SRR33638149/SRR33638149.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR336/053/SRR33638153/SRR33638153.fastq.gzprimaryOK200GenomicsNew England Biolabs Inc.https://www.ebi.ac.uk/ena/browser/view/PRJNA1265467This work aims to solve the age-old mystery of the physiological target of the OLD (overcoming lysogenization defect) nuclease of phage P2, which interferes with the replication of phage Lambda in P2 lysogens. We screened a library of 245 unique phage-encoded tRNAs in E. coli MG1655 engineered to recapitulate the growth defect induced by P2-OLD. To identify rescue tRNAs, next-generation sequencing was run on cells transformed with the phage tRNA library before ("input") and after activation of P2-OLD ("output").ENAfalseATPase-powered antiphage nuclease with tRNA stem-severing activity2025-05-222025-05-22PRJNA1265467