ENAGenomicsImmuno-Oncology, Obstetrics&Gynecology, University Medical Center Groningenhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1268950Homo sapiensPolymerase epsilon mutant (POLE-mut) endometrial cancers (EC) are characterized by a near 100% disease-specific survival rate, even when treated by surgery alone. This spectacular survival, combined with the ultramutated genome and high level of neoantigens in these tumors, indicates a substantial degree of immune control in preventing disease spread and recurrence. Although these features are intriguing, the immune infiltration of POLE-mut EC has predominantly been confined to immunohistochemistry studies. Here, we used state of the art single-cell RNA and TCR sequencing to characterize the immune landscape of POLE-mutant ECs. Moreover, we uniquely analyzed patient blood samples taken two to eight years after curative treatment to assess formation of long-term immune memory in circulation. We identified specialized tumor-infiltrating myeloid subsets at different stages of maturation, an array of lymphocytes ranging from immature to cytotoxic and adaptive natural killer (NK) as well as tumor-reactive exhausted and effector T cells, contributing to a highly inflammatory anti-tumor response. Remarkably, our analysis of blood samples taken years after curative treatment uncovered the presence of tumor-reactive T cell clones that matched the primary tumor. This indicates the formation of systemic long-term memory immune responses in POLE-mut EC survivors. Our study highlights the distinctive immunogenicity of POLE-mut EC and identifies key features associated with persistent anti-tumor immunity that may contribute to prolonged, relapse-free survival. Overall design: Intratumoral immune cells of 4 patients with a POLE mutant endometrial cancer were isolated by Fluorescence-activated cell sorting (FACS) according to the presence or abscence of CD45, CD3, CD8, CD4 and CD19 and analyzed using scRNAseq and scTCRseq.ENAfalseSpatialtemporal immune landscape and long-term immune memory in POLE-mutant endometrial cancer at single cell levelSpatialtemporal immune landscape and long-term immune memory in POLE-mutant endometrial cancer at single cell level2025-09-242025-07-20PRJNA1268950GSE2983209606