{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Department of Pathology and Laboratory Medicine, University of Wisconsin"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA129773"],"scientific_name":["Homo sapiens"],"tag":["xref:PubMed:21112566"],"long_description":["Mesenchymal stem/stromal cells (MSCs) are multipotent cells that can differentiate into a variety of cell types forming connective tissue and skeleton, and are essential participants in the development of all organs. However, MSC precursors remain largely unknown. In human embryonic stem cells (hESCs) directed to mesendodermal differentiation through coculture with OP9 stromal cells, we identified a population of mesodermal cells by surface expression of apelin receptor (APLNR1). APLNR+ cells were enriched with precursors generating compact spheroid colonies in semisolid suspension culture. Being formed by single cells, these colonies consisted of a uniform population of mesenchymal cells with a transcriptional profile representative of embryonic mesenchyme originating from lateral plate/extraembryonic mesoderm. Mesenchymal colony formation required serum-free medium and FGF2 as a colony-forming factor, could be significantly enhanced by PDGF-BB, but suppressed by VEGF. When transferred to the adherent cultures in serum-free medium with FGF2, individual colonies gave rise to multipotential mesenchymal cell lines with typical phenotype (CD146+CD105+CD73+CD31-CD43-CD45-), differentiation (chondro-, osteo-, and adipogenesis) and proliferation (>80 doublings) potentials. Consistent with lineage-restricted differentiation pattern, neither endothelial nor hematopoietic cells could be produced from adherent mesenchymal cultures, however endothelial cells could be derived from mesenchymal colonies in the early days of colony-forming culture suggesting that mesenchymal cells arose from cells with primary angiogenic potential (mesangioblasts). Together these studies identified mesangioblasts as the earliest clonogenic mesenchymal precursors at this stage of their specification from mesoderm. Overall design: This set (8 samples) of expression data is a time-course experiment of hESC (H1) differentiated in OP9 coculture for 1-7 days."],"repository":["ENA"],"description_synonyms":["SCR, Lateral Plate Mesoderm, conditioned mesenchymal stem cell, Mesoderm, l(3)84Af, Experiment., Stromal, Myor, Effects, Lateral Plate Mesoderms, Dorsal, axis, Mesoderms, ABF1, Longterm Effect, Intermediate, stalk, Scanning Transmission, precursor, ABF-1, Plate Mesoderms, Intermediate Mesoderm, Cell, Long Term, bone marrow stromal cells, Electron Microscopy, scr, entire mesoderm, colony-forming unit-fibroblast, MYOR, MyoR, Stromal Cell, Long Term Effects, bHLHa22, Scanning Transmission Electron Microscopy, Paraxial Mesoderms, Dorsal Mesoderms, BHLHA22, primary stem, Effect, parent ion, DmelCG1030, mesodermal mantle, Mesenchyme, BMSC, Mesenchyma, DmScr, Longterm, BG:DS07876.2, Long-Term, Dorsal Mesoderm, Lateral Plate, Plate Mesoderm, MSC, Msc, culm, Longterm Effects, Lateral, Mesenchymas, precursor ion, primary axis, Paraxial, Cells, STEM, marrow stromal cells, embryonic mesoderm, Intermediate Mesoderms, CG1030, Long-Term Effect, Long-Term Effects, Paraxial Mesoderm"],"name_synonyms":["Human, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern Man"],"additional_accession":[]},"is_claimable":false,"name":"Homo sapiens","description":"A mesoderm-derived mesenchymal stem/stromal cells (MSC) precursor: time course experiment","dates":{"last_updated":"2025-09-24","first_public":"2014-02-11"},"accession":"PRJNA129773","cross_references":{"GEO":["GSE20045"],"taxon":["9606"],"PubMed":["21112566"]}}