{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Medical School, Huzhou University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA1300441"],"scientific_name":["Homo sapiens"],"long_description":["This study aims to identify immune-related genes in Autoimmune Hepatitis (AIH) by analyzing liver tissue transcriptomes. Bulk RNA sequencing was performed on liver biopsies from patients with AIH and two disease control groups: Primary Biliary Cholangitis (PBC) and Chronic Hepatitis B (CHB). Differential expression analysis was used to compare AIH against both control groups. By intersecting the resulting differentially expressed genes (DEGs) with immune gene databases, we identified 19 common immune-related DEGs (IRDEGs). Gene Ontology (GO) analysis of these genes highlighted functions related to lymphocyte-mediated immunity and T-cell receptor signaling. This dataset provides a resource for exploring liver gene expression changes in AIH. Overall design: Overall Design: Bulk RNA sequencing was used to compare liver tissue transcriptomes from three patient cohorts: Autoimmune Hepatitis (AIH), Primary Biliary Cholangitis (PBC), and Chronic Hepatitis B (CHB). Sample Processing and Sequencing: Total RNA was isolated from residual liver biopsy tissues. Strand-specific libraries were prepared using the Illumina TruSeq Stranded Total RNA kit and sequenced on an Illumina NovaSeq 6000 platform. Data Analysis: Differentially expressed genes (DEGs) between the AIH group and the two control groups (PBC and CHB) were identified using the DESeq2 R package. The significance threshold was set at a p-value  1."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptome analysis of liver tissue from patients with Autoimmune Hepatitis, Primary Biliary Cholangitis, and Chronic Hepatitis B","description":"Transcriptome analysis of liver tissue from patients with Autoimmune Hepatitis, Primary Biliary Cholangitis, and Chronic Hepatitis B","dates":{"last_updated":"2025-09-24","first_public":"2025-08-07"},"accession":"PRJNA1300441","cross_references":{"GEO":["GSE304352"],"taxon":["9606"]}}