{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["University of Florida"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA1300595"],"long_description":["Microbial biomanufacturing is increasingly recognized as a critical strategy for in situ resource utilization in space exploration, yet it has not been experimentally demonstrated in space, and the effects of microgravity on engineered metabolism remain poorly understood. Here, we systematically evaluate how real and simulated reduced-gravity conditions affect growth and beta-carotene biosynthesis in Saccharomyces cerevisiae, using a genetically engineered production strain and its wild-type parent. Biomanufacturing performance was assessed under simulated Martian, Lunar, and microgravity conditions on Earth, as well as aboard the International Space Station. Simulated microgravity reduced both growth and carotenoid yields, whereas spaceflight experiments revealed enhanced beta-carotene production in the engineered strain. Transcriptomic profiling uncovered gravity-dependent responses, including upregulation of MAPK signaling, fatty acid and sterol biosynthesis, and zinc-responsive IZH genes. Notably, zinc limitation further increased carotenoid yields 3- to 4-fold under simulated normal and microgravity. These findings provide foundational insights into microbial adaptation to space and inform strategies for optimizing biomanufacturing beyond Earth."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"","description":"Gravitational conditions affect beta-carotene production in Saccharomyces cerevisiae","dates":{"last_updated":"2025-08-05","first_public":"2025-08-05"},"accession":"PRJNA1300595","cross_references":{}}