ENAGenomicsPeking University - Third School of Clinical Medicine: Peking University Third Hospitalhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1313185we generated a FHOD3 knockout (FHOD3-/-) human embryonic stem cell (hESC) cell line (H9) by CRISPR/Cas9 system and differentiate it into cardiomyocytes to elucidate the mechanism of cardiomyopathy and HF caused by FHOD3 deficiency. Our study found that FHOD3-deficient hPSC-CMs presented sarcomere disorganization and degradation. In addtion, impaired calcium homeostasis aggravated the pathogenesis of myocardial dysfunction and HF.ENAfalsefhod3 deficiency leads to cardiomyopathy in hESC-CMs2025-09-082025-09-08PRJNA1313185