ENAGenomicsHuazhong Agricultural Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1315184The C2 domain-containing protein family (C2DPs) have been functionally identified in regulating intracellular protein transport and signal transduction in the abiotic stress responses in plants. However, the small C2 domain protein subfamily has not been identified in chilling tolerance in rice. Here, we identified a small C2 domain protein OsERG1, which negatively regulated chilling tolerance in rice seedlings. Additionally, through the screening of yeast two-hybrid cDNA library, we obtained an ABA receptor, OsPYL10, interacted with OsERG1 in a Ca2+ and ABA-dependent manner in vitro and in vivo. Transgenics experiments identified the positive regulation of OsPYL10 to chilling tolerance in rice seedlings.ENAfalseThe C2 domain-containing and Ca2+-binding protein OsERG1 interferes with OsPYL10-OsPP2C09 module to negatively regulate the chilling tolerance in rice2025-09-082025-09-08PRJNA1315184