ENAGenomicsNorthwest A&F Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA1320678To determine the effects of ZmLHT1 inactivation on SLB resistance, we used CRISPR/Cas9-mediated gene editing to generate ZmLHT1 CRISPR/Cas9 knockout lines in the B73 background, Maize seedlings were grown in a greenhouse at 26 deg C with 16 h light and 8 h dark to 4,5 leaf stage. The fourth leaves of zmlht1 mutant and WT plants were inoculated with a suspension of Cochliobolus heterostrophus spores. Control plants were sprayed with sterile distilled water. After inoculation, the plants were kept at 80% humidity for 2 h.Total RNA was extracted using Plant RNA Kit (Omega Biotek, USA) according to the manufacturer protocol. First-strand cDNA was synthesized using the FastKing RT Kit (Tiangen, China) according to the manufacturer protocol.ENAfalseRNA-seq data of zmlht1 mutant and WT seeding leaves in response to southern leaf blight2025-09-082025-09-08PRJNA1320678