{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Bali Pulendran, Emory Vaccine Center, Emory University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA133875"],"scientific_name":["Mus musculus"],"long_description":["Many successful vaccines induce persistent antibody responses that can last a lifetime. The mechanisms by which they do so remain unclear, but emerging evidence suggests that activate dendritic cells (DCs) via Toll-like receptors (TLRs). For example, the yellow fever vaccine YF-17D, one of the most successful empiric vaccines ever developed, activates DCs via multiple TLRs to stimulate pro-inflammatory cytokines. Triggering specific combinations of TLRs in DCs can induce synergistic production of cytokines, which results in enhanced T cell responses, but its impact on antibody responses remain unknown. Learning the critical parameters of innate immunity that programs such antibody responses remains a major challenge in vaccinology. We demonstrated that immunization of mice with synthetic nanoparticles containing antigens plus Toll-like receptor (TLR) ligands 4 (MPL) + 7 (R837) induces synergistic increases in antigen-specific, neutralizing antibodies compared to immunization with a single TLR ligand. To determine whether there was any early programming of B cells, we isolated isotype switched, TCRbeta-CD11b-CD19+IgD-IgG+ B cells by FACS at 7 days post immunization with nanoparticles containing various adjuvants plus OVA, and performed microarray analyses to assess their molecular signatures. Overall design: Two independent sets of samples at day 7 post-treatment were used in our analyses. Each set is comprised by B-cells from mice treated with MPL + R837 or from those treated with either individual MPL or R837 alone."],"tag":["xref:PubMed:21350488"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Mus musculus","description":"Sorted B cells from mice treated with MPL and/or R837","dates":{"last_updated":"2025-09-24","first_public":"2014-02-11"},"accession":"PRJNA133875","cross_references":{"GEO":["GSE25677"],"taxon":["10090"],"PubMed":["21350488"]}}