ENAGenomicsMultiomicsUniversity of Michiganhttps://www.ebi.ac.uk/ena/browser/view/PRJNA136955Homo sapiensSystemic lupus erythematosus (SLE) is characterized by increased vascular risk due to premature atherosclerosis independent of traditional risk factors. We previously proposed that interferon-α plays a crucial role in premature vascular damage in SLE. IFN-α alters the balance between endothelial cell apoptosis and vascular repair mediated by endothelial progenitor cells (EPCs) and myeloid circulating angiogenic cells (CACs). Here we demonstrate that IFN-α promotes an antiangiogenic signature in SLE and control EPCs/CACs, characterized by transcriptional repression of IL-1α and β, IL-1 receptor 1 and vascular endothelial growth factor A (VEGF-A) and upregulation of IL-1 receptor antagonist (IL-1RN) and the decoy receptor IL1-R2. IL-1β promotes significant improvement in the functional capacity of lupus EPCs/CACs, therefore abrogating the deleterious effects of IFN-α. We used microarrays to analyze the effect of IFNα on peripheral blood EPCs/CACs and on bone marrow EPCs exposed to proangiogenic stimulation. Overall design: Human lupus EPCs and CACs from PBMCs were isolated and cultured under proangiogenic stimulation after IFNa incubation or not, RNA was extracted and processed for hybridization on Affymetrix microarrays.xref:PubMed:20805419xref:PubMed:22058412ENAdata, Disease, lupus erythematosus, human being, Libman Sacks Disease, Man (Taxonomy), Systemic, lupus, SLE, Lupus Erythematosus Disseminatus, Modern, susceptibility to, ER-PM contact site, Lupus Erythematosus, endoplasmic reticulum-plasma membrane contact junction., man, human, Human, LIBMAN SACKS DIS, Homo sapiens, Libman-Sacks Disease, ER-plasma membrane contact site, Modern Man, excess LMW-DNA, Libman-Sacks, lupus nephritis, Man, EPCS, excess lymphocyte low molecular weight DNAHuman, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManfalseHomo sapiensExpression data from human lupus EPCs/CACs2025-09-242014-02-11PRJNA136955GSE2695096062080541922058412