ENAGenomicsMultiomicsUniversity of Michiganhttps://www.ebi.ac.uk/ena/browser/view/PRJNA136957Homo sapiensSystemic lupus erythematosus (SLE) is characterized by increased vascular risk due to premature atherosclerosis independent of traditional risk factors. We previously proposed that interferon-α plays a crucial role in premature vascular damage in SLE. IFN-α alters the balance between endothelial cell apoptosis and vascular repair mediated by endothelial progenitor cells (EPCs) and myeloid circulating angiogenic cells (CACs). Here we demonstrate that IFN-α promotes an antiangiogenic signature in SLE and control EPCs/CACs, characterized by transcriptional repression of IL-1α and β, IL-1 receptor 1 and vascular endothelial growth factor A (VEGF-A) and upregulation of IL-1 receptor antagonist (IL-1RN) and the decoy receptor IL1-R2. IL-1β promotes significant improvement in the functional capacity of lupus EPCs/CACs, therefore abrogating the deleterious effects of IFN-α. We used microarrays to analyze the effect of IFNα on peripheral blood EPCs/CACs and on bone marrow EPCs exposed to proangiogenic stimulation. Overall design: Human healthy EPCs from bone marrow were isolated and cultured under proangiogenic stimulation after IFNa incubation or not, RNA was extracted and processed for hybridization on Affymetrix microarrays.xref:PubMed:20805419xref:PubMed:22058412ENAYellow Marrow, data, human being, Man (Taxonomy), medullary bone, medulla of bone, STGD4, Modern, Antigen AC133 homolog, MCDR2, ER-PM contact site, Prominin-like protein 1, endoplasmic reticulum-plasma membrane contact junction., Prom-1, man, PROM1, AC133, human, RP41, antigen AC133 homolog, CD133, Red Marrow, Human, prominin-like protein 1, Prom, antigen AC133, Antigen AC133, Homo sapiens, PROML1, medulla ossium, CORD12, ER-plasma membrane contact site, Modern Man, Marrow, medulla ossea, MSTP061, Red, 4932416E19Rik, Yellow, Man, Proml1, Bone, EPCSHuman, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManfalseHomo sapiensExpression data from human healthy CD133+ bone marrow EPCs2025-09-242014-02-11PRJNA136957GSE2695196062080541922058412