ENAGenomicsMultiomicsDivision of Transgenic Animal Science, Advanced Science Research Center,, Kanazawa Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA137345Mus musculusxref:PubMed:21778144We compared the gene expression profiles of the HP1 gamma -/- vs wild-type PGCs to examine the molecular mechanisms of HP1g in PGC development. Although HP1g is suggested to be involved in PGC proliferation, our microarray data indicated that HP1g does not directly control PGC cell-cycle progression by regulating cell cycle-related genes. However, many genes acting on differentiated tissues were up-regulated in the HP1g-/- PGCs. Overall design: The mVenus-positive cells from the E11.5 genital ridges were isolated by flow cytometry (JSAN, Bay Bioscience). The total RNA was then prepared from the sorted primordial germ cells (about 500-3,500 cells per embryo) of wild-type or HP1γ-/- embryos with an RNeasy micro kit (Qiagen). The RNA samples were pooled from 5 or 6 embryos of each genotype. Two independent pooled RNA samples of each genotypes were used to verify the reproducibility of the microarray analyses.ENAGametes, Germ-Line Cells, HECH, Germ Line, Germ Lines., HP1Hs-gamma, Cells, Gamete, Germ Line Cells, Germ Cell, Hp1-gamma, Germ-Line Cell, Germ, HP1-GAMMA, Cell, Germ-LineMus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscarisfalseMus musculusThe effect of HP1-gamma deficiency on primordial germ cells2025-09-242014-02-11PRJNA137345GSE272941009021778144