ENAGenomicsMultiomicsResearch Core Unit Genomics, Medical School Hannoverhttps://www.ebi.ac.uk/ena/browser/view/PRJNA137997Mus musculusWe investigated the transcriptional profile of B cells isolated from peripheral lymph nodes of CCR7-/-C57BL/6 and C57BL/6 mice respectively. B cells were sorted as follows: CD19+, CD3-, CD11c-, CD11b-, NK1.1-, GR-1-. Very few genes were differentially regulated in CCR7-/- B cells, however the expression of a number of genes associated with B-cell activation was increased in CCR7-/- B cells compared to WT B cells. Our data suggest a role of CCR7 signaling in B-cell activation processes. Overall design: 12 purified RNA samples (6 samples of each genotype), originated from 12 individually facs-sorted cell samples, were used for the microarray study. 3 RNA samples per genotype were pooled, giving rise to 2 wildtype- and 2 Ccr7 knock-out pools. In each of the 2 dual-color microarray hybridizations, samples from 1 wildtype and 1 Ccr7 knock-out pool were cohybridized. Microarray hybridizations 1 and 2 were performed in a dye-swap approach.xref:PubMed:21969271ENA3.4.22.-, B Cells, Node, B Lymphocytes, Laboratory, Bursa-Dependent Lymphocytes, Mus domesticus, mouse, CMKBR7, A4, mini-ICE, CASP-14, CCR7, House Mouse, TYPE, LGMD2C, Cmkbr7, DAGA4, CC-CKR-7, House, Mus, Lymph Nodes, Mini-ICE, chemokine receptor CCR7, Mus musculus domesticus, MIP-3 beta receptor, Cdw197, MAM, SCG3, Mice, Mus musculus., Lymph, C-C CKR-7, CD197, Mus musculus, B-cell, EBI1, DMDA1, Caspase-14 subunit p10, Lymph Node, Swiss, mice, CDw197, Swiss Mouse, CCR-7, Bursa-Equivalent Lymphocyte, House Mice, Swiss Mice, Caspase-14 subunit p19, Ebi1h, EVI1, MICE, Laboratory Mice, domesticus, DMDA, B lymphocyte, B-Lymphocyte, bacterial transcription, Nodes, SCARMD2, ccr7-a, epstein-Barr virus-induced G-protein coupled receptor 1, lymph gland, Mouse, BLR2, B-lymphocyte, transcription from bacterial-type RNA polymerase promoter, Laboratory Mouse, EBV-induced G-protein coupled receptor 1Mus musculus, Laboratory Mice., House, Mus, Laboratory, Swiss, Mus domesticus, mouse, Mus musculus domesticus, Swiss Mouse, mouse <Mus musculus>, Mouse, House Mice, Swiss Mice, house mouse, Mice, Laboratory Mouse, House Mouse, mice C57BL/6xCBA/CaJ hybrid, domesticus, Mus muscarisfalseMus musculusTranscription profile of B cells isolated from peripheral lymph nodes of wild-type vs CCR7-/- mice2025-09-242014-02-11PRJNA137997GSE278851009021969271