ENAGenomicsMultiomicsProbiogenomics Lab., Dept. Genetics, University of Parmahttps://www.ebi.ac.uk/ena/browser/view/PRJNA144187Bifidobacterium bifidumIn this study, we investigated the transcriptome of Bifidobacterium bifidum PRL2010 during in vitro growth by micro array technology. When B. bifidum PRL2010 was grown in liquid broth, 425 of the 1644 PRL2010 genes represented on the array were expressed in at least one of the three investigated growth phases, i.e., lag-, exponential and stationary phase. These transcriptional analyses identified a core in vitro transcriptome encompassing 150 genes, which resulted expressed in all phases. A proportion of the latter genes were further investigated as potential reference genes by Quantitative Real Time PCR (qRT-PCR) assays. Their expression stability was evaluated under different growth conditions, encompassing cultivation on different carbon sources, exposure to environmental stresses (thermal, acidic and osmotic) and growth phases. Our analyses validated six reference genes suitable for normalizing mRNA expression levels in qRT-PCR experiments applied to bifidobacteria. Overall design: Transcriptional profiling of B.bifidum PRL2010 at different growth phases (lag phase, early exponential phase, late exponential phase, early stationary phase).xref:PubMed:22003014ENAAnchored Polymerase Chain Reaction, Materials, Transcriptome, determination, Effects, Transcriptome Profile, Lactobacillus parabifidus, Expression Profiles, number, Longterm Effect, Gene Expression Profile, Gene, Polymerase Chain Reactions, Actinomyces parabifidus, Profiles, Inverse, Cistrons, Nocardia bifida, presence, Long Term, Bacillus bifidus communis, whole transcriptome, Gene Expression, count in organism, Inverse PCR, Cohnistreptothrix bifidus, Expression Signatures, Long Term Effects, Gene Expression Signatures, Actinobacterium bifidum, Genetic Materials, Gene Expression Signature, Polymerase Chain, Lactobacillus bifidus type II, Effect, Expression Profile, Bacillus bifidus, Genetic Material, PCR, Transcriptome Profiles, Anchored, Genetic, Reactions, Nested, Nested Polymerase Chain Reaction, Bacterium bifidum, Longterm, Profile, Inverse Polymerase Chain Reaction, chemical analysis., Bifidibacterium bifidum, Long-Term, Actinomyces bifidus, Signatures, Longterm Effects, polymerase chain reaction, Material, Expression Signature, Gene Expression Profiles, Reaction, Tissieria bifida, Transcriptomes, Nested PCR, Cistron, Long-Term Effect, Anchored PCR, assay, Signature, Long-Term Effects, Bacteroides bifidusCohnistreptothrix bifidus, Bacterium bifidum, Tissieria bifida, Actinobacterium bifidum, Lactobacillus parabifidus, Bifidibacterium bifidum, Lactobacillus bifidus type II, Actinomyces parabifidus, Bacillus bifidus communis., Bacillus bifidus, Nocardia bifida, Actinomyces bifidus, Bacteroides bifidusfalseBifidobacterium bifidumIdentification of reference genes for quantitative Real-Time PCR in Bifidobacterium bifidum PRL2010 based on transcriptome analysis2025-09-242014-02-11PRJNA144187GSE30832168122003014