ENAGenomicsMultiomicsOmid Khorram, OB-GYN, LA Biomed at UCLA medical centerhttps://www.ebi.ac.uk/ena/browser/view/PRJNA169086Homo sapiensmircoRNA-200c (miR-200c) through repression of specific target genes has been associated with cellular transition, tumorigenesis and tissue fibrosis. We explored the expression and functional aspects of miR-200c in genesis of leiomyomas, benign uterine tumors with fibrotic characteristic. Gain-of function of miR-200c in isolated leiomyoma and myometrial smooth muscle cells (LSMC and MSMC) and leiomyosarcoma cell line (SKLM-S1) repressed ZEB1/ZEB2 mRNAs and proteins, with concurrent increase in E-cadherin (CDH1) and reduction in vimentin expression, phenotypic alteration and inhibition of MSMC and LSMC proliferation. We further validated TIMP2, FBLN5 and VEGFA as direct targets of miR-200c through interaction with their respective 3’UTRs, and other genes as determined by microarray analysis. Overall design: Total RNA isolated from MSMC and LSMC following gain-of function of miR-200c was subjected to gene expression profiling using HumanHT-12 v4 Expression BeadChip (Illumina, Inc. San Diego, CA) consists of 47,000 oligonucleotide probe sets representing 28,688 transcripts.xref:PubMed:22685266ENA9430057C20Rik, HH, TIMP-2, l(3)hh, ARCL1A, Vegf164, Vegf120, Myosin regulatory light chain interacting protein, EVEC., 6.3.2.-, ARMD3, bar, TIMP2, IDOL, CG4637, Hh, CG12249, A55, AW228700, FIBL-5, tissue inhibitor of metalloproteinases 2, KIAA1731NL, anon-WO0134654.19, DmelCG4637, VEGF-A, bar3, DmelCG12249, Mir, MIR, vegf-a, VEGF164, Miranda, MVCD1, Vegf, l(3)neo56, l(3)neo57, MONDOA, Vegf188, DDC8, Timp-2, VPF, Vpf, Mrt, Mira, DANCE, UP50, D11Bwg1104e, ADCL2, Inducible degrader of the LDL-receptor, mir, CSC-21K, Myosin regulatory light chain-interacting protein, VEGF, anon-WO0182946.19, bar-3, MIRA, VEGFA, bHLHe36, vegf, Idol, vegfa, hgHuman, Modern., human being, Man (Taxonomy), Homo sapiens, man, Man, human, Modern ManfalseHomo sapiensmiR-200c is Aberrantly Expressed in Leiomyomas in Ethnic-Dependent Manner and Targets ZEBs, VEGFA, TIMP2 and FBLN52025-09-242014-02-11PRJNA169086GSE38817960622685266